Electroporation and dielectrophoresis of single cells using a microfluidic system employing a microwave interferometric sensor

Author

Salimi, E. ; Nikolic-Jaric, Marija ; Cabel, Tim ; Braasch, Katrin ; Jung, Volker ; Butler, Mairead ; Thomson, D. ; Bridges, Greg E.

Author_Institution

Dept. of Electr. & Comput., Univ. of Manitoba, Winnipeg, MB, Canada

fYear

2013

fDate

2-7 June 2013

Firstpage

1

Lastpage

4

Abstract

We describe a microfluidic device integrated with a microwave interferometric sensor that is able to simultaneously electroporate and measure the dielectrophoresis (DEP) response of single biological cells. The system can measure changes in dielectric properties of a cell permeabilized using a high-intensity pulsed electric field (PEF) from a few seconds after exposure to the pulses. It provides a mechanism for investigating time-dependent changes in the cell membrane and ion flux. Using this device, experiments performed on single Chinese hamster ovary (CHO) cells exposed to microsecond pulsed electric fields show significant changes in their DEP response immediately after exposure.

Keywords

bioelectric phenomena; biomembranes; cellular biophysics; electrophoresis; microfluidics; Chinese hamster ovary cells; cell membrane; dielectrophoresis; electroporation; high intensity pulsed electric field; ion flux; microfluidic system; microwave interferometric sensor; single biological cells; time dependent changes; Biomembranes; Conductivity; Dielectrics; Electric fields; Electrodes; Force; Microfluidics; CHO; dielectrophoresis; electroporation; microfluidic; microwave interferometer; pulsed electric field;

fLanguage

English

Publisher

ieee

Conference_Titel

Microwave Symposium Digest (IMS), 2013 IEEE MTT-S International

Conference_Location

Seattle, WA

ISSN

0149-645X

Print_ISBN

978-1-4673-6177-4

Type

conf

DOI

10.1109/MWSYM.2013.6697715

Filename

6697715