Hexamethylene-1,3-bis(cetyldimethylammonium bromide) protected low-toxicity ZnSe quantum dots as fluorescent probe for proteins

Author

Yaping Zhong ; Chun Deng ; Yu He ; Yili Ge ; Gongwu Song

Author_Institution

Minist.-of-Educ. Key Lab. for the Synthesis & Applic. of Org. Functional Mol., Hubei Univ., Wuhan, China

Volume

10

Issue

5

fYear

2015

fDate

5 2015

Firstpage

236

Lastpage

240

Abstract

Hexamethylene-1,3-bis(cetyldimethylammonium bromide) (G_16-6-16) protected low-toxicity ZnSe quantum dots (QDs) were successfully synthesised and used as fluorescence probe to detect proteins such as bovine serum albumin (BSA). This method is simple, fast, selective and sensitive. Surfactant G_16-6-16 can interact with proteins through hydrophobic effect, thus leading to the fluorescence quenching of G_16-6-16 protected ZnSe QDs nanometre material. The fluorescence intensity of G_16-6-16 protected ZnSe QDs was linearly proportional to BSA over a concentration range from 0 to 80 nM with a correlation coefficient of 0.993. The detection limit was 0.04 nM. This method was successfully applied to determine BSA in urine samples which shows the good application value of this nanometre material.

Keywords

II-VI semiconductors; biochemistry; fluorescence; molecular biophysics; nanosensors; optical sensors; proteins; radiation quenching; semiconductor quantum dots; surfactants; wide band gap semiconductors; zinc compounds; bovine serum albumin; correlation coefficient; detection limit; fluorescence intensity; fluorescence quenching; fluorescent probe; hexamethylene-1,3-bis(cetyldimethylammonium bromide); hydrophobic effect; low-toxicity ZnSe quantum dots; proteins; surfactant G_16-6-16 protected ZnSe QD nanometre material;

fLanguage

English

Journal_Title

Micro & Nano Letters, IET

Publisher

iet

ISSN

1750-0443

Type

jour

DOI

10.1049/mnl.2014.0698

Filename

7108345