Multiple micro-contact printing of extra cellular matrix with fine alignment

Author

Tanaka, Nobuyuki ; Ota, Hiroyuki ; Fukumori, Kazuhiro ; Yamato, Masayuki ; Okano, Teruo

Author_Institution

Instn. for Adv. Biomed. Sci., Tokyo Women´s Med. Univ., Tokyo, Japan

fYear

2013

fDate

10-13 Nov. 2013

Firstpage

1

Lastpage

2

Abstract

This study performed multiple micro-contact printing of extra-cellular matrix with a fine alignment system for the position and the posture of polydimethylsiloxane (PDMS) stamp. The position and the posture were adjusted with a 4-axis automated stage with a resolution of 1 μm or 0.0025 deg. The adjustment was achived by monitoring the surface shape of PDMS stamp measured by using a laser displacement sensor in the alignment system. After the adjustment, fluorescent-dye-conjugated fibronectin applied on the surface of PDMS stamp was printed onto a cell culture dish. As the result of multiple printing, two different patterns of fibronectin were located on the same dish within an error of 50 μm.

Keywords

biosensors; cellular biophysics; displacement measurement; dyes; fluorescence; laser applications in medicine; molecular biophysics; optical sensors; polymers; proteins; soft lithography; 4-axis automated stage; PDMS stamp; cell culture dish; extracellular matrix; fine alignment system; fluorescent-dye-conjugated fibronectin; laser displacement sensor; multiple microcontact printing; polydimethylsiloxane stamp position; polydimethylsiloxane stamp posture; surface shape monitoring; Accuracy; Displacement measurement; Fluorescence; Measurement by laser beam; Printing; Shape measurement; Substrates;

fLanguage

English

Publisher

ieee

Conference_Titel

Micro-NanoMechatronics and Human Science (MHS), 2013 International Symposium on

Conference_Location

Nagoya

Print_ISBN

978-1-4799-1527-9

Type

conf

DOI

10.1109/MHS.2013.6710393

Filename

6710393