Compiled chip for all pretreatment processes of virus gene analysis

Author

Niimi, Michiharu ; Masuda, T. ; Kaihatsu, Kunihiro ; Kato, Nei ; Arai, Fumihito

Author_Institution

Nagoya Univ., Nagoya, Japan

fYear

2013

fDate

10-13 Nov. 2013

Firstpage

1

Lastpage

2

Abstract

In this research, we demonstrated a microfluidic chip to pretreat the samples for viral genome assay. The microfluidic chip has the following three functions; (1) Virus purification and enrichment, (2) Viral RNA extraction, and (3) Capture of the targeted virus genome. Hydroxyapatite chromatography, Boom method, and PNA (Peptide Nucleic Acid) were used for the above three functions, respectively. These three functions were integrated in one chip. Furthermore PNA immobilized on the glass can detect the targeted virus genome so that in situ virus detection would be possible by anybody, anywhere, anytime.

Keywords

RNA; biosensors; chromatography; genetics; genomics; lab-on-a-chip; microfluidics; microorganisms; microsensors; molecular biophysics; proteins; purification; Boom method; SiO₂; compiled chip; glass; hydroxyapatite chromatography; in situ virus detection; microfluidic chip; peptide nucleic acid; pretreatment processes; targeted virus genome capture; viral RNA extraction; viral genome assay; virus enrichment; virus gene analysis; virus purification; Bioinformatics; Genomics; Glass; Influenza; Microfluidics; Purification; RNA;

fLanguage

English

Publisher

ieee

Conference_Titel

Micro-NanoMechatronics and Human Science (MHS), 2013 International Symposium on

Conference_Location

Nagoya

Print_ISBN

978-1-4799-1527-9

Type

conf

DOI

10.1109/MHS.2013.6710394

Filename

6710394