Title :
Sensitivity compensation of multi-fluorescence sensor toward on-chip cell measurement
Author :
Hengjun Liu ; Maruyama, Hisataka ; Masuda, T. ; Arai, Fumihito
Author_Institution :
Dept. of Micro-Nano Syst. Eng., Nagoya Univ., Nagoya, Japan
Abstract :
We synthesized a novel multi-fluorescence sensor which can respond to both pH and temperature. This kind of sensor was based on amino-polystyrene microbeads and two different fluorescent indicators (Rhodamine B and FITC) were applied simultaneously to synthesize the multi-sensor. Rhodamine B shows an excellent linear relationship between the relative fluorescent intensity and temperature while it is independent to PH. The relative fluorescence intensity of FITC can respond to both pH and temperature. We succeeded in the compensation of the pH sensitivity of FITC using temperature measurement by Rhodamine B.
Keywords :
biosensors; cellular biophysics; dyes; fluorescence; lab-on-a-chip; microsensors; optical sensors; pH; pH measurement; polymers; proteins; sensitivity; temperature measurement; FITC; Rhodamine B; amino-polystyrene microbeads; fluorescent indicators; multifluorescence sensor; on-chip cell measurement; pH response; pH sensitivity; relative fluorescent intensity; sensitivity compensation; temperature measurement; temperature response; Calibration; Equations; Fluorescence; Polymers; Sensitivity; Temperature measurement; Temperature sensors;
Conference_Titel :
Micro-NanoMechatronics and Human Science (MHS), 2013 International Symposium on
Conference_Location :
Nagoya
Print_ISBN :
978-1-4799-1527-9
DOI :
10.1109/MHS.2013.6710415
