Title :
Patterning of human induced pluripotent stem cells using patterned plasma treatment on a PDMS surface followed by composite protein adsorption
Author :
Yamada, Ryota ; Hattori, K. ; Tagaya, Motohiro ; Sasaki, T. ; Sugiura, Shinya ; Kanamori, Toshiyuki ; Ohnuma, Kentaro
Author_Institution :
Dept. of Bioeng., Nagaoka Univ. of Technol., Nagaoka, Japan
Abstract :
The goal of this article is to make a pattern of human induced pluripotent stem cells (hiPSCs) on a Polydimethylsiloxane (PDMS) surface. Here, we report that pattering of hiPSCs can be achieved by patterned plasma treatment followed by coating with a two protein mixture. The result is useful for future applications of micro devices in human cell culture.
Keywords :
adsorption; biochemistry; cellular biophysics; molecular biophysics; patient treatment; polymers; proteins; PDMS surface; composite protein adsorption; hiPSC pattering; human cell culture; human-induced pluripotent stem cells; microdevices; patterned plasma treatment; polydimethylsiloxane surface; protein mixture; Adsorption; Coatings; Immune system; Plasmas; Proteins; Stem cells; Surface treatment;
Conference_Titel :
Micro-NanoMechatronics and Human Science (MHS), 2013 International Symposium on
Conference_Location :
Nagoya
Print_ISBN :
978-1-4799-1527-9
DOI :
10.1109/MHS.2013.6710454
