Ultrasonic Frequency Response Analysis for Quantitative Measurements in Bone Marrow Stromal Cells

Bone tissue engineering techniques have become new approaches in bone regeneration. Before clinical implantation, the preconditioning is needed. Therefore, we implement the ultrasonic evaluation system without cellular destruction. This study focuses the cellular proliferation into the composites of bone marrow stromal cells (BMSCs) / β-tricalcium phosphate (β-TCP) and composes the ultrasonic cell quantity determination on frequency domain for the BMSCs / β-TCP composites after being cultured: 4 types BMSCs to 24 β-TCP scaffolds. This system aims viscous attenuation because viscosity is proportional to frequency-squared. On frequency domain, we confirmed the attenuation in the immediate vicinity of 1.0 MHz, which is the center frequency of the probe. Moreover, it is discussed and concluded, the findings in this work illustrate that the frequency properties of BMSCs / ß-TCP composites have the prominent osteoconductive activity and the potential for applications/approaches in future regenerative medicine.