• DocumentCode
    688625
  • Title

    Red-emitting substrates for rapid botulinum neurotoxin detection

  • Author

    Herron, James N. ; Engelhardt, Konrad H. ; O´Mel, Vlad

  • Author_Institution
    Dept. of Pharm. & Pharm. Chem., Univ. of Utah, Salt Lake City, UT, USA
  • fYear
    2013
  • fDate
    9-14 June 2013
  • Firstpage
    1
  • Lastpage
    2
  • Abstract
    We report a new red-emitting molecular beacon for botulinum neurotoxin A suitable inexpensive rapid assay formats. Reaction kinetics including parameters like Km and Vmax were evaluated in a fluorogenic assay using the BoNT/A light chain.
  • Keywords
    biochemistry; biosensors; enzymes; fluorescence; molecular biophysics; molecular configurations; optical sensors; reaction kinetics; sensor arrays; toxicology; BoNT/A light chain; botulinum neurotoxin A; fluorogenic assay; inexpensive rapid assay formats; rapid botulinum neurotoxin detection; reaction kinetics; red-emitting molecular beacon; red-emitting substrates; Biomedical optical imaging; Fluorescence; Kinetic theory; Optical sensors; Proteins; Substrates;
  • fLanguage
    English
  • Publisher
    ieee
  • Conference_Titel
    Lasers and Electro-Optics (CLEO), 2013 Conference on
  • Conference_Location
    San Jose, CA
  • Type

    conf

  • Filename
    6833000
    • Link To Document
    https://search.isc.ac/dl/search/defaultta.aspx?DTC=49&DC=688625