Title :
Imaging DNA damage-dependent chromatin dynamics via nonlinear photoperturbation
Author :
Tomas, Mark ; Blumhardt, P. ; Deutzmann, A. ; Kromm, D. ; Leitenstorfer, Alfred ; Ferrando-May, E.
Author_Institution :
Dept. of Phys., Univ. of Konstanz, Konstanz, Germany
Abstract :
We employ near-infrared femtosecond pulses to probe local alterations of chromatin dynamics caused by DNA strand breaks. Nonlinear confocal nanomanipulation enables us to visualize how DNA damage signaling emanates spatio-temporally in a live cell.
Keywords :
DNA; cellular biophysics; fluorescence; molecular biophysics; molecular configurations; optical microscopy; proteins; DNA damage signaling visualization; DNA damage-dependent chromatin dynamics imaging; DNA strand breaks; live cell; local alterations; near-infrared femtosecond pulses; nonlinear confocal nanomanipulation; nonlinear photoperturbation; spatio-temporal signaling; DNA; Fluorescence; Frequency conversion; Imaging; Maintenance engineering; Proteins; Ultrafast optics;
Conference_Titel :
Lasers and Electro-Optics (CLEO), 2013 Conference on
Conference_Location :
San Jose, CA
