Title :
High-speed live-cell super-resolution microscopy with stochastically switching fluorophores
Author :
Fang Huang ; Hartwich, Tobias M. P. ; Rivera-Molina, Felix E. ; Yu Lin ; Myers, Jordan R. ; Long, Jane J. ; Davidson, Michael W. ; Toomre, D. ; Bewersdorf, Joerg
Author_Institution :
Sch. of Med., Dept. of Cell Biol., Yale Univ., New Haven, CT, USA
Abstract :
Super-resolution microscopy with stochastically blinking fluorophores (FPALM/PALM/STORM, etc.) achieves ~25 nm resolution by localizing molecules in thousands of camera frames. Here I present a solution to speed up recording dramatically, enabling nanoscopy at video rate.
Keywords :
biomedical optical imaging; cellular biophysics; optical microscopy; FPALM; PALM; STORM; camera frames; fluorescence photoactivation localization microscopy; high-speed live-cell superresolution microscopy; nanoscopy; stochastically blinking fluorophores; stochastically switching fluorophores; video rate; Educational institutions; Fluorescence; Microscopy; Optical diffraction; Optical microscopy; Spatial resolution;
Conference_Titel :
Lasers and Electro-Optics (CLEO), 2013 Conference on
Conference_Location :
San Jose, CA
