Experimental analysis of two menthods for aptamer immobilization on love-wave aptasensors

Author

Li-na Cheng ; Hong-lang Li ; Ya-bing Ke ; Shi-tang He ; Zhao-yang Tong

Author_Institution

Lab. of Acoust. Micro-Electr. Mech. Syst., Inst. of Acoust., Beijing, China

fYear

2013

fDate

25-27 Oct. 2013

Firstpage

1

Lastpage

3

Abstract

The impacts of two immobilization methods, which are Biotin-avidin system (BAS) and Self-assembled monolayer technology (SAM), made on the performance of aptasensors in terms of acoustics were analyzed through experiment. Love-wave aptasensors were designed by coupling aptamers to the surface of a Love-wave sensor with the two methods respectively. Abrin is chosen as the analyte. For certain concentrations of Abrin changed from 10ng/ml to 1ug/ml, the reaction time of BAS and SAM were almost 20 minutes, and the real-time frequency shift of these two methods were quite similar. It is concluded that the Love-wave detectors can remedy the defect of SAM technology.

Keywords

Love waves; biochemistry; biological specimen preparation; biosensors; chemical analysis; molecular biophysics; monolayers; proteins; real-time systems; self-assembly; surface acoustic wave sensors; BAS reaction time; Love wave aptasensor design; Love wave detectors; SAM reaction time; SAM technology defect; abrin concentrations; acoustics; analyte selection; aptamer coupling; aptamer immobilization; biotin-avidin system; immobilization method effect; love wave aptasensor performance; real-time frequency shift; self-assembled monolayer technology; Biosensors; Probes; Real-time systems; Surface acoustic waves; Time-frequency analysis; Abrin; Aptasensors; BAS; Love-wave; SAM;

fLanguage

English

Publisher

ieee

Conference_Titel

Piezoelectricity, Acoustic Waves and Device Applications (SPAWDA), 2013 Symposium on

Conference_Location

Changsha

Print_ISBN

978-1-4799-3289-4

Type

conf

DOI

10.1109/SPAWDA.2013.6841110

Filename

6841110