A Method for Quantitative Determination of the Number of Magnetosomes in Magnetotactic Bacteria by a Spectrophotometer

Author

Tao Song ; Luze Zhao ; Long-Fei Wu

Author_Institution

Beijing Key Lab. of Bioelectromagnetism, Inst. of Electr. Eng., Beijing, China

Volume

50

Issue

11

fYear

2014

fDate

Nov. 2014

Firstpage

1

Lastpage

4

Abstract

A method for quantitative determination of the number of magnetosomes in magnetotactic bacteria (MTB) was developed in this paper. Using a magnetism measuring system that composed of a common laboratory spectrophotometer and an electromagnetic system, the relation between the R_mag of the MTB solution and the magnetic flux density of the applied magnetic field could be measured. The formula to calculate the percentage distribution of the bacteria with different numbers of magnetosomes was deduced in this paper, which depended on the R_mag curve under different magnetic flux densities. The related parameters were obtained by fitting the real measured results. Finally, the distribution of the MTB with different numbers of magnetosomes was detected by the proposed method.

Keywords

biomagnetism; biomembranes; electromagnetism; magnetic flux; microorganisms; spectrophotometers; MTB solution; R_mag curve; applied magnetic field; common laboratory spectrophotometer; electromagnetic system; magnetic flux density; magnetism measuring system; magnetosomes; magnetotactic bacteria; percentage distribution; quantitative determination; Coils; Density measurement; Magnetic resonance imaging; Magnetic separation; Microorganisms; Superconducting magnets; Torque; Magnetic field; magnetosome; magnetotactic bacteria (MTB); spectrophotometer;

fLanguage

English

Journal_Title

Magnetics, IEEE Transactions on

Publisher

ieee

ISSN

0018-9464

Type

jour

DOI

10.1109/TMAG.2014.2323953

Filename

6971518