Development and characterization of a pulsatile-flow bioreactor for Vascular Graft studies

Author

Mansouri, Wasila ; Rindone, Alexandra N. ; Cooper, James A.

Author_Institution

Dept. of Biomed. Eng., Rensselaer Polytech. Inst., Troy, NY, USA

fYear

2015

fDate

17-19 April 2015

Firstpage

1

Lastpage

2

Abstract

Tissue Engineered Vascular Grafts aim to mimic the physiological environment, structure, and function of a native vessel. In order to investigate the effects of a poly(□-caprolactone) (PCL) biomimetic graft, a bioreactor system was developed to provide pressurized, pulsatile flow through the graft. The bioreactor system was calibrated for flow and pressure profiles to target physiological shear stresses on the walls of the lumen of the graft. In future studies, the bioreactor system will allow for dynamic co-culture of smooth muscle cells and endothelial cells on the PCL bilayer vascular graft.

Keywords

biomechanics; biomedical equipment; biomimetics; bioreactors; blood vessels; calibration; cellular biophysics; internal stresses; materials preparation; muscle; polymers; prosthetics; pulsatile flow; tissue engineering; PCL bilayer vascular graft; PCL biomimetic graft effect; bioreactor system calibration; dynamic cell coculture; endothelial cell culture; flow profile; graft lumen wall; native vessel function mimicking; native vessel physiological environment mimicking; native vessel structure mimicking; polycaprolactone biomimetic graft effect; pressure profile; pressurized pulsatile flow; pulsatile-flow bioreactor characterization; pulsatile-flow bioreactor development; smooth muscle cell culture; target physiological shear stress; tissue engineered vascular graft; Arteries; Electron tubes; Muscles; Physiology; Stress; Tissue engineering;

fLanguage

English

Publisher

ieee

Conference_Titel

Biomedical Engineering Conference (NEBEC), 2015 41st Annual Northeast

Conference_Location

Troy, NY

Print_ISBN

978-1-4799-8358-2

Type

conf

DOI

10.1109/NEBEC.2015.7117116

Filename

7117116