Title :
Development and characterization of a pulsatile-flow bioreactor for Vascular Graft studies
Author :
Mansouri, Wasila ; Rindone, Alexandra N. ; Cooper, James A.
Author_Institution :
Dept. of Biomed. Eng., Rensselaer Polytech. Inst., Troy, NY, USA
Abstract :
Tissue Engineered Vascular Grafts aim to mimic the physiological environment, structure, and function of a native vessel. In order to investigate the effects of a poly(□-caprolactone) (PCL) biomimetic graft, a bioreactor system was developed to provide pressurized, pulsatile flow through the graft. The bioreactor system was calibrated for flow and pressure profiles to target physiological shear stresses on the walls of the lumen of the graft. In future studies, the bioreactor system will allow for dynamic co-culture of smooth muscle cells and endothelial cells on the PCL bilayer vascular graft.
Keywords :
biomechanics; biomedical equipment; biomimetics; bioreactors; blood vessels; calibration; cellular biophysics; internal stresses; materials preparation; muscle; polymers; prosthetics; pulsatile flow; tissue engineering; PCL bilayer vascular graft; PCL biomimetic graft effect; bioreactor system calibration; dynamic cell coculture; endothelial cell culture; flow profile; graft lumen wall; native vessel function mimicking; native vessel physiological environment mimicking; native vessel structure mimicking; polycaprolactone biomimetic graft effect; pressure profile; pressurized pulsatile flow; pulsatile-flow bioreactor characterization; pulsatile-flow bioreactor development; smooth muscle cell culture; target physiological shear stress; tissue engineered vascular graft; Arteries; Electron tubes; Muscles; Physiology; Stress; Tissue engineering;
Conference_Titel :
Biomedical Engineering Conference (NEBEC), 2015 41st Annual Northeast
Conference_Location :
Troy, NY
Print_ISBN :
978-1-4799-8358-2
DOI :
10.1109/NEBEC.2015.7117116
