Title :
SNR characterization of Mesoscopic Fluorescence Molecular Tomography with EMCCD camera
Author :
Ji, Robin ; Ozturk, Mehmet S. ; Intes, Xavier
Author_Institution :
Dept. of Biomed. Eng., Rensselaer Polytech. Inst., Troy, NY, USA
Abstract :
Progress in bio-printing techniques has accentuated the need for non-invasive imaging modalities with high sensitivity, high resolution, and imaging depth of a few millimeters. Mesoscopic Fluorescence Molecular Tomography (MFMT) is a promising imaging modality for 3D localization and quantification of fluorescent labeled cells in thick scaffolds. Here we report on the characterization of our second generation MFMT system which uses an Electron-Multiplying charge coupled device (EMCCD). More precisely, we report on the effects of EM gain and deep cooling of the camera on signal-to-noise ratio (SNR) and hence the sensitivity. Experimental results showed that these parameters, when used in conjunction, increased SNR by at least 3 fold.
Keywords :
CCD image sensors; biomedical optical imaging; fluorescence; image denoising; image resolution; medical image processing; optical tomography; 3D localization; 3D quantification; EM gain effects; EMCCD camera; SNR characterization; bioprinting techniques; deep cooling; electron-multiplying charge coupled device; fluorescent labeled cells; high resolution; imaging depth; imaging modality; mesoscopic fluorescence molecular tomography; noninvasive imaging modality; second generation MFMT system; signal-noise ratio; Cooling; Detectors; Fluorescence; Gain; Imaging; Signal to noise ratio; EMCCD; fluorescence imaging; laminar optical tomography; mesoscopic imaging; signal-to-noise ratio;
Conference_Titel :
Biomedical Engineering Conference (NEBEC), 2015 41st Annual Northeast
Conference_Location :
Troy, NY
Print_ISBN :
978-1-4799-8358-2
DOI :
10.1109/NEBEC.2015.7117169
