SNR characterization of Mesoscopic Fluorescence Molecular Tomography with EMCCD camera

Author

Ji, Robin ; Ozturk, Mehmet S. ; Intes, Xavier

Author_Institution

Dept. of Biomed. Eng., Rensselaer Polytech. Inst., Troy, NY, USA

fYear

2015

fDate

17-19 April 2015

Firstpage

1

Lastpage

2

Abstract

Progress in bio-printing techniques has accentuated the need for non-invasive imaging modalities with high sensitivity, high resolution, and imaging depth of a few millimeters. Mesoscopic Fluorescence Molecular Tomography (MFMT) is a promising imaging modality for 3D localization and quantification of fluorescent labeled cells in thick scaffolds. Here we report on the characterization of our second generation MFMT system which uses an Electron-Multiplying charge coupled device (EMCCD). More precisely, we report on the effects of EM gain and deep cooling of the camera on signal-to-noise ratio (SNR) and hence the sensitivity. Experimental results showed that these parameters, when used in conjunction, increased SNR by at least 3 fold.

Keywords

CCD image sensors; biomedical optical imaging; fluorescence; image denoising; image resolution; medical image processing; optical tomography; 3D localization; 3D quantification; EM gain effects; EMCCD camera; SNR characterization; bioprinting techniques; deep cooling; electron-multiplying charge coupled device; fluorescent labeled cells; high resolution; imaging depth; imaging modality; mesoscopic fluorescence molecular tomography; noninvasive imaging modality; second generation MFMT system; signal-noise ratio; Cooling; Detectors; Fluorescence; Gain; Imaging; Signal to noise ratio; EMCCD; fluorescence imaging; laminar optical tomography; mesoscopic imaging; signal-to-noise ratio;

fLanguage

English

Publisher

ieee

Conference_Titel

Biomedical Engineering Conference (NEBEC), 2015 41st Annual Northeast

Conference_Location

Troy, NY

Print_ISBN

978-1-4799-8358-2

Type

conf

DOI

10.1109/NEBEC.2015.7117169

Filename

7117169