Integrated optofluidic device for the measurement of the activity of lymphocytes

Author

Usuba, R. ; Yokokawa, M. ; Llobera, A. ; Murata, S. ; Ohkohchi, N. ; Suzuki, H.

Author_Institution

Grad. Sch. of Pure & Appl. Sci., Univ. of Tsukuba, Tsukuba, Japan

fYear

2015

fDate

21-25 June 2015

Firstpage

261

Lastpage

264

Abstract

To measure activity of lymphocytes by cytokine detection, an optofluidic device with integrated optical components and a flow channel structure for the processing of solutions created with poly(dimethylsiloxane) (PDMS) was developed. Absorbance measurement was conducted effectively with integrated lenses and mirrors. An air bypass formed in the flow channel structure was effective to exchange solutions in a measuring chamber avoiding the introduction of air. A cytokine was detected using the optofluidic device. Total assay time was less than 30 min and cytokine was detected in pg/mL level.

Keywords

biomedical equipment; cellular biophysics; integrated optoelectronics; lab-on-a-chip; microfluidics; proteins; absorbance measurement; cytokine detection; flow channel structure; integrated optofluidic device; lymphocyte activity measurement; poly(dimethylsiloxane); Fluid flow measurement; Immune system; Microfluidics; Optical devices; Optical variables measurement; Plugs; Sea measurements; Drug susceptibility test; cytokine; lymphocytes; on-chip immunoassay; optofluidics;

fLanguage

English

Publisher

ieee

Conference_Titel

Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS), 2015 Transducers - 2015 18th International Conference on

Conference_Location

Anchorage, AK

Type

conf

DOI

10.1109/TRANSDUCERS.2015.7180911

Filename

7180911