Shape Anisotropic Magnetic Particles for high throughput and high efficiecy intracelluar delivery of functional macromolecules

Author

Lin, M.Y. ; Wu, Y.C. ; Lee, J.A. ; Zhou, J. ; Teitell, M.A. ; Chiou, P.Y.

Author_Institution

Nat. Appl. Res. Labs., Instrum. Technol. Res. Center, Taiwan

fYear

2015

fDate

21-25 June 2015

Firstpage

880

Lastpage

883

Abstract

We report a novel intracellular delivery system based on aligning Shape-Anisotropic Magnetic Particles (SAMP) to deliver functional molecules into mammalian cells with high efficiency and high viability. A broad range of molecules (calcein, dextran 3K, dextran 40K, GFP plasmid, SiRNA, and β-lactamase) have been successfully delivered into primary cells with preserved functions. We have achieved high efficiency delivery of enzyme into primary human dermal fibroblast (NHDF), and other large macromolecules (dextran 40K) into mouse cortical neurons.

Keywords

biomagnetism; cellular biophysics; drug delivery systems; enzymes; magnetic particles; β-lactamase; GFP plasmid; SiRNA; calcein; dextran 3K; dextran 40K; enzyme; functional macromolecules intracellular delivery; mammalian cells; mouse cortical neurons; primary human dermal fibroblast; shape anisotropic magnetic particles; Biochemistry; Fibroblasts; Magnetic particles; Mice; Neurons; Perpendicular magnetic anisotropy; Intracellular delivery; SiRNA; functional macromolecules; shape-anisotropic magnetic particles;

fLanguage

English

Publisher

ieee

Conference_Titel

Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS), 2015 Transducers - 2015 18th International Conference on

Conference_Location

Anchorage, AK

Type

conf

DOI

10.1109/TRANSDUCERS.2015.7181064

Filename

7181064