Measurement of reactive oxygen species release from stimulated cell culture with fully integrated microsensor system by advanced electrochemical detection principle

A novel electrochemical sensing system for the monitoring of reactive oxygen species (ROS) from drug stimulated mouse fibroblast cell culture is presented. The sensing approach utilizes a direct oxidation of the analyte superoxide on polymer membrane covered gold microelectrodes. The sensor performance was compared to state of the art sensors based on cytochrome c (Cyt c) and superoxide dismutase (SOD) as recognition elements. Outstanding higher sensor sensitivity was shown, as well as sufficient long term stability and good selectivity towards the main interfering substances uric acid and hydrogen peroxide. The electrochemical microsensor was integrated into a standard 6-well plate, which enables convenient cell cultivation for at least 24 h and successive superoxide monitoring from drug stimulated cell culture without the need of cell transfer.