D2O based microfluidics for in vitro ir cell analysis

Author

Ebrahimifard, R. ; van den Driesche, S. ; Lang, W. ; Vellekoop, M.J.

Author_Institution

Microsyst. Center Bremen (MCB), Univ. of Bremen, Bremen, Germany

fYear

2015

fDate

21-25 June 2015

Firstpage

1688

Lastpage

1691

Abstract

We present the use of D₂O (deuterium oxide) as a liquid medium for infrared analysis of biological cells in microfluidic devices. In contradiction to standard buffer liquids, D₂O shows a low infrared absorption in the range of 3.3 to 3.6 μm wavelength, which makes it suitable for infrared analysis methods in that range. We illustrate the performance by the measurement of CH₂ and CH₃ stretch vibrations of phospholipid bilayer of the cell membrane of yeast, in a microfluidic chip.

Keywords

bioMEMS; biological techniques; biomembranes; cellular biophysics; heavy water; infrared spectroscopy; lab-on-a-chip; lipid bilayers; microfluidics; microorganisms; molecular biophysics; vibrational states; CH₂ stretch vibration measurement; CH₃ stretch vibration measurement; D₂O; D₂O based microfluidics; biological cell analysis; cell membrane phospholipid bilayer; deuterium oxide liquid medium; in vitro IR cell analysis; infrared analysis; microfluidic chip; standard buffer liquid; wavelength 3.3 mum to 3.6 mum; wavelength range; yeast; Absorption; Biomembranes; Microfluidics; Semiconductor device measurement; Vibrations; Water; D2O; cells; infrared sensor; microfluidics;

fLanguage

English

Publisher

ieee

Conference_Titel

Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS), 2015 Transducers - 2015 18th International Conference on

Conference_Location

Anchorage, AK

Type

conf

DOI

10.1109/TRANSDUCERS.2015.7181268

Filename

7181268