Title :
Continuous lactate monitoring by microsensors in spheroid 3D tumor cell cultures
Author :
Weltin, A. ; Hammer, S. ; Kaminski, Y. ; Klein, S. ; Noor, F. ; Kieninger, J. ; Urban, G.A.
Author_Institution :
Dept. of Microsyst. Eng. - IMTEK, Univ. of Freiburg, Freiburg, Germany
Abstract :
This paper reports the combination of an electrochemical metabolic monitoring system with a spheroid 3D tumor cell culture environment. Precise lactate production from single human hepatocyte spheroids was continuously monitored online over hours by electrochemical microsensors, also in combination with drug exposure. The approach enhances and simplifies the determination of metabolic rates from organotypic 3D cell cultures in drug discovery and toxicology.
Keywords :
bioMEMS; biochemistry; biomedical equipment; cellular biophysics; drugs; electrochemical sensors; microsensors; organic compounds; toxicology; tumours; continuous lactate monitoring; drug discovery; drug exposure; drug toxicology; electrochemical metabolic monitoring system; electrochemical microsensor; lactate production; metabolic rate determination; online monitoring; organotypic 3D cell culture; single human hepatocyte spheroid; spheroid 3D tumor cell culture environment; Biosensors; Drugs; Electrodes; Monitoring; Production; Three-dimensional displays; 3D cell culture; lactate; microsensors; monitoring; spheroids; toxicology;
Conference_Titel :
Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS), 2015 Transducers - 2015 18th International Conference on
Conference_Location :
Anchorage, AK
DOI :
10.1109/TRANSDUCERS.2015.7181270
