Title :
Floating-flower inspired cell culture platform for simplifying medium exchange based on elasto-capillarity
Author :
Hong, H. ; Park, S.J. ; Na, M.H. ; Park, S.M. ; Kim, D.S.
Author_Institution :
Dept. of Mech. Eng., POSTECH, Pohang, South Korea
Abstract :
In this study, Flower-Shaped Polydimethylsiloxane (PDMS) Substrate (FSPS) motivated from a floating flower was proposed to simplify a cell culture experiment for studying an effect of cell microenvironments. The FSPS can capture and dispense a cell culture medium, forming a pocket by physical interaction between an elastic substrate and the medium. Thus, we can exchange the medium with simple lifting up and pushing down actions without repetitive pipetting. The developed platform was verified to substitute a conventional PDMS substrate utilized in the studies, through stability and sustainability of the medium pocket, and NIH3T3 cell proliferation experiments.
Keywords :
bioMEMS; biological techniques; capillarity; cellular biophysics; microfluidics; polymers; substrates; FSPS; NIH3T3 cell proliferation experiment; cell culture experiment; cell culture medium capture; cell culture medium dispensing; cell microenvironment effect; conventional PDMS substrate; elastic substrate-medium physical interaction; elasto-capillarity; floating-flower inspired cell culture platform; flower-shaped polydimethylsiloxane substrate; medium exchange simplification; medium pocket stability; medium pocket sustainability; Assembly; Curing; Substrates; Surface morphology; Surface topography; Surface treatment; Cell culture substrate; Cell microenvironment; Floating flower; Medium exchange;
Conference_Titel :
Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS), 2015 Transducers - 2015 18th International Conference on
Conference_Location :
Anchorage, AK
DOI :
10.1109/TRANSDUCERS.2015.7181311
