Title :
FRET-based scanning probe microscopy with a donor dye coated AFM tip
Author :
Kalanoor, Basanth S. ; Berezin, Shirly ; Kapon, Omree ; Tischler, Yaakov R.
Author_Institution :
Dept. of Chem., Bar-Ilan Univ., Ramat-Gan, Israel
Abstract :
A FRET-based method of near-field fluorescence lifetime imaging is developed. Lumogen dye attached to the apex of an AFM tip acts as a nanometeric light source for exciting the sample via non-radiative energy transfer.
Keywords :
atomic force microscopy; dyes; fluorescence; near-field scanning optical microscopy; optical images; FRET-based method; FRET-based scanning probe microscopy; Lumogen dye; donor dye coated AFM tip; nanometeric light source; near-field fluorescence lifetime imaging; nonradiative energy transfer; Excitons; Fluorescence; Image resolution; Microscopy; Optical imaging; Optical microscopy;
Conference_Titel :
Lasers and Electro-Optics (CLEO), 2015 Conference on
Conference_Location :
San Jose, CA
