Title :
Dynamic label-free imaging of live-cell adhesion using photonic crystal enhanced microscopy (PCEM)
Author :
Yue Zhuo ; Ji Sun Choi ; Hojeong Yu ; Harley, Brendan A. C. ; Cunningham, Brian T.
Author_Institution :
Dept. of Bioeng., Univ. of Illinois at Urbana-Champaign, Urbana, IL, USA
Abstract :
We demonstrate label-free imaging of cell attachment upon a photonic-crystal biosensor surface. Newly-implemented PCEM image-analysis software is used to dynamically visualize individual live-cell movement and demonstrate the spatiotemporal-distribution of cellular material during adhesion and motion.
Keywords :
adhesion; biological techniques; biomechanics; biosensors; cell motility; optical images; optical microscopy; optical sensors; photonic crystals; cell attachment; cellular material; dynamic label-free imaging; live-cell adhesion; live-cell movement; photonic crystal enhanced microscopy; photonic-crystal biosensor surface; spatiotemporal-distribution; Adhesives; Biosensors; Image color analysis; Imaging; Optical surface waves; Stem cells;
Conference_Titel :
Lasers and Electro-Optics (CLEO), 2015 Conference on
Conference_Location :
San Jose, CA
