Title :
An azimuthal polarizer assures localization accuracy in single-molecule super-resolution fluorescence microscopy
Author :
Lew, Matthew D. ; Moerner, W.E.
Author_Institution :
Depts. of Chem., Stanford Univ., Stanford, CA, USA
Abstract :
Imaging only azimuthally-polarized light from single fluorescent molecules avoids emission from the z component of their transition dipole moments, resulting in accurate measurement of location regardless of emitter orientation and degree of objective lens misfocus.
Keywords :
biomedical optical imaging; fluorescence; lenses; molecular biophysics; molecular moments; optical microscopy; optical polarisers; azimuthal polarizer; emitter orientation; localization accuracy; objective lens misfocus; single-molecule super-resolution fluorescence microscopy; transition dipole moments; Accuracy; Fluorescence; Image resolution; Lenses; Microscopy; Optical microscopy;
Conference_Titel :
Lasers and Electro-Optics (CLEO), 2015 Conference on
Conference_Location :
San Jose, CA
