Title :
Adaptive optics in three-photon fluorescence microscopy
Author :
Sinefeld, David ; Paudel, Hari P. ; Ouzounov, Dimitre G. ; Bifano, Thomas G. ; Xu, Chris
Author_Institution :
Sch. of Appl. & Eng. Phys., Cornell Univ., Ithaca, NY, USA
Abstract :
We demonstrate three-photon fluorescence adaptive-optics system based on 1660-nm femtosecond pulses and MEMS spatial light modulator. We use the higher nonlinearity of the signal resulting in ×700 improvement in fluorescence beads signal after aberrations correction.
Keywords :
aberrations; adaptive optics; fluorescence; high-speed optical techniques; micro-optomechanical devices; multiphoton spectra; nonlinear optics; optical microscopy; spatial light modulators; MEMS spatial light modulator; aberration correction; femtosecond pulses; fluorescence bead signal; three-photon fluorescence adaptive-optics system; three-photon fluorescence microscopy; wavelength 1660 nm; Adaptive optics; Fluorescence; Image resolution; Microscopy; Optical microscopy; Scattering;
Conference_Titel :
Lasers and Electro-Optics (CLEO), 2015 Conference on
Conference_Location :
San Jose, CA
