A Si-chip-based system for highly parallel electroporation of cells

Author

Kupfer, Frank ; Lattanzio, Silvia M. ; Maschietto, Marta ; Botos, Andras ; Mahnkopf, Marlies ; Bruns, Jurgen ; Schreiter, Matthias ; Vassanelli, Stefano ; Thewes, Roland

Author_Institution

Fac. of Electr. Eng. & Comput. Sci., Tech. Univ. Berlin, Berlin, Germany

fYear

2015

fDate

18-19 June 2015

Firstpage

25

Lastpage

30

Abstract

A single-cell electroporation system is presented capable for efficient, highly parallel, and noninvasive transfection of biological cells as required in cloning experiments. The 2D geometry of Si-chips with cells cultivated on their surface enables good controllability and accessibility of the experiment using standard microscopic monitoring methods. On the basis of a modified silicon-on-insulator (SOI) technology chips are processed with 192 electroporation sites. The assembled chips are operated in a setup which allows application of a large variety of electrical protocols. First results from cell culture experiments prove feasibility.

Keywords

bioelectric phenomena; cellular biophysics; lab-on-a-chip; silicon-on-insulator; 2D geometry; accessibility; biological cells; cell parallel electroporation; controllability; silicon-on-insulator technology; single cell electroporation; standard microscopic monitoring methods; Dielectrics; Electrodes; Monitoring; Protocols; Silicon; Standards; Surface treatment; electroporation; silicon-on-insulator (SOI); transfection;

fLanguage

English

Publisher

ieee

Conference_Titel

Advances in Sensors and Interfaces (IWASI), 2015 6th IEEE International Workshop on

Conference_Location

Gallipoli

Type

conf

DOI

10.1109/IWASI.2015.7184936

Filename

7184936