Title :
A Si-chip-based system for highly parallel electroporation of cells
Author :
Kupfer, Frank ; Lattanzio, Silvia M. ; Maschietto, Marta ; Botos, Andras ; Mahnkopf, Marlies ; Bruns, Jurgen ; Schreiter, Matthias ; Vassanelli, Stefano ; Thewes, Roland
Author_Institution :
Fac. of Electr. Eng. & Comput. Sci., Tech. Univ. Berlin, Berlin, Germany
Abstract :
A single-cell electroporation system is presented capable for efficient, highly parallel, and noninvasive transfection of biological cells as required in cloning experiments. The 2D geometry of Si-chips with cells cultivated on their surface enables good controllability and accessibility of the experiment using standard microscopic monitoring methods. On the basis of a modified silicon-on-insulator (SOI) technology chips are processed with 192 electroporation sites. The assembled chips are operated in a setup which allows application of a large variety of electrical protocols. First results from cell culture experiments prove feasibility.
Keywords :
bioelectric phenomena; cellular biophysics; lab-on-a-chip; silicon-on-insulator; 2D geometry; accessibility; biological cells; cell parallel electroporation; controllability; silicon-on-insulator technology; single cell electroporation; standard microscopic monitoring methods; Dielectrics; Electrodes; Monitoring; Protocols; Silicon; Standards; Surface treatment; electroporation; silicon-on-insulator (SOI); transfection;
Conference_Titel :
Advances in Sensors and Interfaces (IWASI), 2015 6th IEEE International Workshop on
Conference_Location :
Gallipoli
DOI :
10.1109/IWASI.2015.7184936
