Moveable Wire Electrode Microchamber for Nanosecond Pulsed Electric-Field Delivery

Author

Yu-Hsuan Wu ; Arnaud-Cormos, D. ; Casciola, M. ; Sanders, J.M. ; Leveque, P. ; Vernier, P.T.

Author_Institution

Mork Family Dept. of Chem. Eng. & Mater. Sci., Univ. of Southern California, Los Angeles, CA, USA

Volume

60

Issue

2

fYear

2013

fDate

Feb. 2013

Firstpage

489

Lastpage

496

Abstract

In this paper, an electromagnetic characterization of a moveable wire electrode microchamber for nanosecond pulse delivery is proposed. The characterization of the exposure system was carried out through experimental measurements and numerical simulations. The frequency and time domain analyses demonstrate the utility of the proposed assembly for delivering pulses as short as 2.5 ns. High-voltage measurements (~1.2 kV) were also performed using pulse generators based on two different technologies with applied pulse durations of 5.0 and 2.5 ns. Validation of the delivery system was accomplished with biological experiments involving cell electroporation with 2.5 and 5.0 ns, 10-MV/m pulsed electric fields. A dose-dependent area increase (osmotic swelling) of the Jurkat cells was observed with pulses as short as 2.5 ns.

Keywords

bioelectric phenomena; biomedical electrodes; blood; cancer; cellular biophysics; microelectrodes; numerical analysis; osmosis; swelling; time-frequency analysis; voltage measurement; Jurkat cells; applied pulse durations; biological experiments; cell electroporation; dose-dependent area; electromagnetic characterization; experimental measurements; exposure system; frequency-time domain analysis; high-voltage measurements; moveable wire electrode microchamber; nanosecond pulsed electric-field delivery; numerical simulations; osmotic swelling; pulse generators; time 2.5 ns; time 5.0 ns; Electric fields; Electrodes; Generators; Impedance; Tungsten; USA Councils; Wires; Cell electroporation; finite difference time domain (FDTD); high voltage; nanosecond pulsed electric field (nsPEF); wire electrodes; Cell Size; Computer Simulation; Electrodes; Electromagnetic Fields; Electroporation; Humans; Jurkat Cells; Microtechnology; Tungsten;

fLanguage

English

Journal_Title

Biomedical Engineering, IEEE Transactions on

Publisher

ieee

ISSN

0018-9294

Type

jour

DOI

10.1109/TBME.2012.2228650

Filename

6359787