Preliminary electrochemical characterisation of cytochrome P4501A2-clozapine interaction

Author

Antonini, M. ; Ghisellini, P. ; Pastorino, L. ; Paternolli, C. ; Nicolini, C.

Author_Institution

Dept. of Biophys. M&O Sci. & Technol., Univ. of Genoa, Italy

Volume

150

Issue

1

fYear

2003

fDate

6/18/2003 12:00:00 AM

Firstpage

31

Lastpage

34

Abstract

Cytochromes P450 are a large superfamily of heme-thiolate enzymes involved in the metabolism of many different organic substrates such as drugs, fatty acids and toxic compounds. The aim of this work is to analyse the binding between the cytochrome P4501A2, in solution and in gel-matrix, and its substrate (clozapine), utilising voltammetric tests. The interaction measurements were carried out using two different screen printed electrodes (rhodium-graphite and graphite-riboflavin), and the results were compared. It was demonstrated that it is possible to realise a biosensor prototype to detect the presence of clozapine indirectly by chronoamperometry.

Keywords

biochemistry; biosensors; electrochemistry; proteins; Rh; binding analysis; biosensor prototype; chronoamperometry; cytochrome P4501A2-clozapine interaction; drugs; electrochemical characterisation; fatty acids; graphite-riboflavin; heme-thiolate enzymes; rhodium-graphite; screen printed electrodes; toxic compounds;

fLanguage

English

Journal_Title

Nanobiotechnology, IEE Proceedings -

Publisher

iet

ISSN

1478-1581

Type

jour

DOI

10.1049/ip-nbt:20030534

Filename

1220705