Gold-nanoparticle-assisted oligonucleotide immobilisation for improved DNA detection

Author

Minard-Basquin, C. ; Kügler, R. ; Matsuzawa, N.N. ; Yasuda, A.

Author_Institution

Stuttgart Technol. Center, Mater. Sci. Lab., Stuttgart, Germany

Volume

152

Issue

2

fYear

2005

fDate

4/8/2005 12:00:00 AM

Firstpage

97

Lastpage

103

Abstract

Colloidal gold nanoparticles are investigated as a potential scaffold for the assisted immobilisation of probe oligonucleotides on silicon surfaces. A preliminary study is devoted to the examination of the immobilisation of DNA-modified gold nanoparticles as a function of time, concentration, salt and pH. The DNA-modified nanoparticles self-assembled onto solid surfaces in a three-dimensional self-assembled architecture. The functionalised surfaces are evaluated in diagnostic assays, where their potential to improve the efficiency of the hybridisation reaction is tested. The system utilising DNA-modified nanoparticles produced an enhancement in the hybridisation efficiency and the sensitivity limit by a factor 10 to 100 as compared to a conventional DNA immobilisation system on a planar surface.

Keywords

DNA; biosensors; colloids; gold; molecular biophysics; nanoparticles; self-assembly; Au; DNA detection; DNA immobilisation system; DNA-modified gold nanoparticles; DNA-modified nanoparticles; assisted immobilisation scaffold; concentration; diagnostic assays; functionalised surfaces; gold-nanoparticle-assisted oligonucleotide immobilisation; hybridisation efficiency; hybridisation reaction; pH; planar surface; probe oligonucleotides; salt; sensitivity limit; solid surface self-assembled; three-dimensional self-assembled architecture;

fLanguage

English

Journal_Title

Nanobiotechnology, IEE Proceedings -

Publisher

iet

ISSN

1478-1581

Type

jour

DOI

10.1049/ip-nbt:20055019

Filename

1425338