DocumentCode :
810698
Title :
Toward nanometer-scale resolution in fluorescence microscopy using spectral self-interference
Author :
Swan, Anna K. ; Moiseev, Lev A. ; Cantor, C.R. ; Davis, Brynmor ; Ippolito, S.B. ; Karl, William Clem ; Goldberg, Bennett B. ; Ünlü, M. Selim
Author_Institution :
Electr. & Comput. Eng. Dept., Boston Univ., MA, USA
Volume :
9
Issue :
2
fYear :
2003
Firstpage :
294
Lastpage :
300
Abstract :
We introduce a new fluorescence microscopy technique that maps the axial position of a fluorophore with subnanometer precision. The interference of the emission of fluorophores in proximity to a reflecting surface results in fringes in the fluorescence spectrum that provide a unique signature of the axial position of the fluorophore. The nanometer sensitivity is demonstrated by measuring the height of a fluorescein monolayer covering a 12-nm step etched in silicon dioxide. In addition, the separation between fluorophores attached to the top or the bottom layer in a lipid bilayer film is determined. We further discuss extension of this microscopy technique to provide resolution of multiple layers spaced as closely as 10 nm for sparse systems.
Keywords :
Langmuir-Blodgett films; biological techniques; fluorescence; image resolution; light interference; light interferometry; lipid bilayers; molecular biophysics; monolayers; optical microscopy; 12 nm; Langmuir-Blodgett films; SiO2-Si; axial position; bottom layer; cell biology; fluorescein monolayer; fluorescence microscopy technique; fluorescence spectrum fringes; fluorophore; interferometric technique; lipid bilayer film; molecular biology; multiple layers; nanometer sensitivity; nanometer-scale resolution; reflecting surface; silicon dioxide; sparse systems; spectral self-interference; subnanometer precision; top layer; Biomedical engineering; Cells (biology); Diffraction; Fluorescence; Interference; Nanobioscience; Optical interferometry; Optical microscopy; Spatial resolution; Stimulated emission;
fLanguage :
English
Journal_Title :
Selected Topics in Quantum Electronics, IEEE Journal of
Publisher :
ieee
ISSN :
1077-260X
Type :
jour
DOI :
10.1109/JSTQE.2003.814191
Filename :
1238993
Link To Document :
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