Optically Controlled Cell Discrimination and Trapping Using Optoelectronic Tweezers

Author

Ohta, Aaron T. ; Chiou, Pei-Yu ; Phan, Huan L. ; Sherwood, Steven W. ; Yang, Joon M. ; Lau, Aldrich N K ; Hsu, Hsan-Yin ; Jamshidi, Arash ; Wu, Ming C.

Author_Institution

Dept. of Electr. Eng. & Comput. Sci., California Univ., Berkeley, CA

Volume

13

Issue

2

fYear

2007

Firstpage

235

Lastpage

243

Abstract

Optoelectronic tweezers (OET) provide a powerful tool for the manipulation of micro- and nanoparticles. The OET device produces an optically controlled dielectrophoretic force, allowing complex dynamic manipulation patterns using light intensities up to 100 000 times lower than that of optical tweezers. Using OET, we demonstrate the separation of live and dead human B cells, and the separation of HeLa and Jurkat cells. We also present, for the first time, a modified single-sided OET device that promises to facilitate the integration of OET and microfluidics. Unlike standard OET, this single-sided OET device produces electric fields that are oriented parallel to the plane of the device. We demonstrate the manipulation of polystyrene beads using this new single-sided OET device, and discuss its capabilities

Keywords

biomedical equipment; cellular biophysics; electrophoresis; microfluidics; nanoparticles; quantum optics; radiation pressure; HeLa cells; Jurkat cells; complex manipulation patterns; dead human B cells; dynamic manipulation patterns; live human B cells; microfluidics; microparticle manipulation; nanoparticle manipulation; optical tweezers; optically controlled cell discrimination; optically controlled cell trapping; optically controlled dielectrophoretic force; optoelectronic tweezers; polystyrene bead manipulation; Charge carrier processes; Dielectrophoresis; Force control; Humans; Lighting control; Manipulator dynamics; Microfluidics; Nanoparticles; Optical control; Optical devices; Dielectrophoresis (DEP); optical tweezers; optically induced dielectrophoresis; optoelectronic tweezers (OET);

fLanguage

English

Journal_Title

Selected Topics in Quantum Electronics, IEEE Journal of

Publisher

ieee

ISSN

1077-260X

Type

jour

DOI

10.1109/JSTQE.2007.893558

Filename

4159992