Predicting Interacting Residues Using Long-Distance Information and Novel Decoding in Hidden Markov Models

Identification of interacting residues involved in protein-protein and protein-ligand interaction is critical for the prediction and understanding of the interaction and has practical impact on mutagenesis and drug design. In this work, we introduce a new decoding algorithm, ETB-Viterbi, with an early traceback mechanism, and apply it to interaction profile hidden Markov models (ipHMMs) to enable optimized incorporation of long-distance correlations between interacting residues, leading to improved prediction accuracy. The method was applied and tested to a set of domain-domain interaction families from the 3DID database, and showed statistically significant improvement in accuracy measured by F-score. To gauge and assess the method´s effectiveness and robustness in capturing the correlation signals, sets of simulated data based on the 3DID dataset with controllable correlation between interacting residues were also used, as well as reversed sequence orientation. It was demonstrated that the prediction consistently improves as the correlations increase and is not significantly affected by sequence orientation.