Stabilizing effect of artificial stabilizers for binding activity of QCM immunosensors

Author

Park, Jong-Won ; Kurosawa, Shigeru ; Aizawa, Hidenobu ; Wakida, Shin-ichi ; Yamada, Satoshi ; Ishihara, Kazuhiko

Author_Institution

Nat. Inst. of Adv. Ind. Sci. & Technol., Japan

Volume

50

Issue

10

fYear

2003

Firstpage

1234

Lastpage

1235

Abstract

We compare stabilizing effects of artificial and natural stabilizers for maintenance of binding activity of immobilized antibody on the gold surface of quartz crystal microbalance (QCM). Binding activity between immobilizing antibody and specific antigen was measured and considered as a calculated frequency shift with QCM-immunosensor after temperature increasing and long-term storage. According to the experimental results, one stabilizer among eight samples shows the best stabilizing effect for QCM-immunosensor.

Keywords

biosensors; crystal resonators; microbalances; quartz; Au; SiO/sub 2/; artificial stabilizer; binding activity; frequency shift; gold surface; immobilized antibody; immunosensor; natural stabilizer; quartz crystal microbalance; specific antigen; Chemicals; Electrodes; Frequency measurement; Gold; Immune system; Laboratories; Pollution measurement; Polymers; Proteins; Temperature; Adsorption; Antigen-Antibody Complex; Biosensing Techniques; Coated Materials, Biocompatible; Drug Stability; Electrochemistry; Equipment Failure Analysis; Immunoassay; Microchemistry; Protein Binding; Quartz; Temperature;

fLanguage

English

Journal_Title

Ultrasonics, Ferroelectrics, and Frequency Control, IEEE Transactions on

Publisher

ieee

ISSN

0885-3010

Type

jour

DOI

10.1109/TUFFC.2003.1244737

Filename

1244737