Title :
Silver nanoparticles synthesised using plant extracts show strong antibacterial activity
Author :
Kumari, Avnesh ; Guliani, Anika ; Singla, Rubbel ; Yadav, Ramdhan ; Yadav, Sudesh Kumar
Author_Institution :
Biotechnol. Div., Inst. of Himalayan Bioresource Technol., Palampur, India
Abstract :
In this study, three plants Populus alba, Hibiscus arboreus and Lantana camara were explored for the synthesis of silver nanoparticles (SNPs). The effect of reaction temperature and leaf extract (LE) concentration of P. alba, H. arboreus and L. camara was evaluated on the synthesis and size of SNPs. The SNPs were characterised by ultra-violet-visible spectroscopy, scanning electron microscopy and atomic force microscopy. The synthesis rate of SNPs was highest with LE of L. camara followed by H. arboreus and P. alba under similar conditions. L. camara LE showed maximum potential of smaller size SNPs synthesis, whereas bigger particles were formed by H. arboreous LE. The size and shape of L. camara LE synthesised SNPs were analysed by transmission electron microscopy (TEM) and energy dispersive X-ray spectroscopy (EDX). TEM analysis revealed the formation of SNPs of average size 17 ± 9.5 nm with 5% LE of L. camara. The SNPs synthesised by LE of L. camara showed strong antibacterial activity against Escherichia coli. The results document that desired size SNPs can be synthesised using these plant LEs at a particular temperature for applications in the biomedical field.
Keywords :
X-ray chemical analysis; antibacterial activity; atomic force microscopy; cellular biophysics; microorganisms; nanofabrication; nanomedicine; nanoparticles; particle size; scanning electron microscopy; silver; transmission electron microscopy; ultraviolet spectra; visible spectra; Ag; EDX; Escherichia coli; H. arboreus; Hibiscus arboreus; L. camara; Lantana camara; P. alba; Populus alba; TEM; antibacterial activity; atomic force microscopy; biomedical field; leaf extract concentration; plant extracts; reaction temperature; scanning electron microscopy; silver nanoparticle size; transmission electron microscopy; ultraviolet-visible spectroscopy;
Journal_Title :
Nanobiotechnology, IET
DOI :
10.1049/iet-nbt.2014.0021