DocumentCode
835359
Title
Magnetic immunoassays utilizing magnetic markers and a high-Tc SQUID
Author
Enpuku, K. ; Inoue, K. ; Soejima, K. ; Yoshinaga, K. ; Kuma, H. ; Hamasaki, N.
Author_Institution
Res. Inst. of Supercond. Sci. & Syst., Kyushu Univ., Fukuoka, Japan
Volume
15
Issue
2
fYear
2005
fDate
6/1/2005 12:00:00 AM
Firstpage
660
Lastpage
663
Abstract
Magnetic immunoassays utilizing magnetic markers and a high-Tc SQUID have been performed. We first showed a design of the SQUID for the sensitive detection of the magnetic signal from the marker, where the spatial distribution of the signal field was taken into account. Using the design, we can obtain the relationship between the magnetization of the maker and the signal flux detected with the SQUID. This relationship is important for quantitative evaluation of the immunoassay. Next, we detected biological materials called IgE with a remanence measurement method, where a marker made of Fe3O4 particles with diameter of 25 nm was used. We showed that 2 atto-mol of IgE can be detected, which shows high sensitivity of the present method.
Keywords
SQUID magnetometers; biomedical measurement; biosensors; ferromagnetic materials; magnetic particles; nanoparticles; remanence; sapphire; 20 nm; Fe3O4; biological materials; high-Tc SQUID; magnetic immunoassays; magnetic markers; magnetic signal detection; remanence measurement method; Biological materials; Immune system; Magnetic field measurement; Magnetic flux; Magnetization; Particle measurements; Remanence; SQUIDs; Signal design; Signal detection; High-; immunoassay; magnetic nanoparticle; remanence;
fLanguage
English
Journal_Title
Applied Superconductivity, IEEE Transactions on
Publisher
ieee
ISSN
1051-8223
Type
jour
DOI
10.1109/TASC.2005.849991
Filename
1439724
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