High content cellular imaging for drug development

This paper discusses the bioinformatics issues related to high content screening (HCS) and presents a bioinformatics pipeline for HCS drug development. The paper reviews the advanced methods for bioimage processing, cell segmentation, neurite centerline extraction, and cell tracking and registration in the context of HCS of different bioassays. These methods prove that HCS is potentially a powerful tool in quantitative cell biology and drug discovery. There remain, however, many challenging issues that require the development of new bioimage analysis algorithms to fulfil the potential of HCS