Title :
Addressing cell-sourcing limitations with gene therapy
Author :
García, Andrés J. ; Guldberg, Robert E. ; Byers, Benjamin A. ; Gersbach, Charles A. ; Phillips, Jennifer E.
Author_Institution :
George W. Woodruff Sch. of Mech. Eng., Georgia Inst. of Technol., Atlanta, GA, USA
Abstract :
Genetic engineering with Run×2/Cbfa 1 for an alternative to biological grafts. Forced Runx2 expression via retroviral gene delivery promotes osteoblastic differentiation and mineralization in bone marrow stromal cells and stimulates conversion of myoblasts and dermal fibroblasts into a mineralizing osteoblastic phenotype. This genetic engineering strategy provides a promising approach to overcome cell-sourcing limitations in bone tissue engineering.
Keywords :
bone; cellular biophysics; genetic engineering; molecular biophysics; patient treatment; proteins; tissue engineering; bone marrow stromal cells; bone tissue engineering; cell-sourcing limitations; dermal fibroblasts; genetic engineering strategy; mineralization; mineralizing osteoblastic phenotype; osteoblastic differentiation; retroviral gene delivery; Biological information theory; Bone tissue; Bovine; Cells (biology); Encoding; Gene expression; Gene therapy; Genetic engineering; Mineralization; Rats; Animals; Bone Marrow Cells; Cell Differentiation; Cells, Cultured; Gene Therapy; Genetic Vectors; Male; Mice; Mice, Inbred BALB C; Myoblasts; Neoplasm Proteins; Osteoblasts; Protein Engineering; Rats; Rats, Wistar; Recombinant Proteins; Stromal Cells; Transcription Factors;
Journal_Title :
Engineering in Medicine and Biology Magazine, IEEE
DOI :
10.1109/MEMB.2003.1256274
