Title :
Microdevices for separation, accumulation, and analysis of biological micro- and nanoparticles
Author :
Kentsch, J. ; Dürr, M. ; Schnelle, T. ; Gradl, G. ; Müller, T. ; Jäger, M. ; Normann, A. ; Stelzle, M.
Author_Institution :
Naturwissenschaftliches und Medizinisches Inst., Reutlingen, Germany
Abstract :
Microfabrication and performance of a novel microsystem for separation, accumulation and analysis of biological micro- and nanoparticles is reported. Versatile chip functions based on dielectrophoresis and microfluidics were integrated to isolate particles from complex sample solutions such as serum. A bead-based assay for virus detection is proposed. Separation of micro- and sub-μm beads employing dielectrophoretic deflector and bandpass structures is demonstrated. Individual antibody coated beads with hepatitis A virus bound to their surface were trapped by negative dielectrophoresis in a field cage and analysed by fluorescence microscopy.
Keywords :
biochemistry; bioelectric phenomena; biosensors; cellular biophysics; electrophoresis; fluorescence; microelectrodes; microfluidics; microorganisms; nanoparticles; optical microscopy; spectrochemical analysis; accumulation; analysis; antibody coated beads; bandpass structures; bead-based assay; biological analytes; biological microparticles; biological nanoparticles; complex sample solutions; dielectrophoresis; dielectrophoretic deflector; field cage; fluorescence microscopy; hepatitis A virus; microdevices; microfabrication; microfluidics; microsystem; negative dielectrophoresis; separation; serum; versatile chip functions; virus detection;
Journal_Title :
Nanobiotechnology, IEE Proceedings -
DOI :
10.1049/ip-nbt:20031127
