Rapid development of nucleic acid diagnostics

Author

Fitch, J. Patrick ; Gardner, Shea N. ; Kuczmarski, Thomas A. ; Kurtz, Stefan ; Myers, Rich ; Ott, Linda L. ; Slezak, Thomas R. ; Vitalis, Elizabeth A. ; Zemla, Adam T. ; Mccready, Paula M.

Author_Institution

Lawrence Livermore Nat. Lab., California Univ., USA

Volume

90

Issue

11

fYear

2002

fDate

11/1/2002 12:00:00 AM

Firstpage

1708

Lastpage

1721

Abstract

There has been a significant increase, fueled by technologies front the human genome project, in the availability of nucleic acid sequence information for viruses and bacteria. This paper presents a computer-assisted process that begins with nucleic acid sequence information and produces highly specific pathogen signatures. When combined with instrumentation using the polymerase chain reaction, the resulting diagnostics are both specific and sensitive. The computational and engineering aspects of converting raw sequence data into pathogen-specific and instrument-ready assays are presented. Examples and data are presented for specific pathogens, including foot-and-mouth disease virus and the human immunodeficiency virus.

Keywords

DNA; diseases; medical diagnostic computing; microorganisms; patient diagnosis; reviews; bacteria; foot-and-mouth disease virus; highly specific pathogen signatures; human genome project; human immunodeficiency virus; nucleic acid diagnostics; nucleic acid sequence information; polymerase chain reaction; raw sequence data conversion; viruses; Bioinformatics; Data engineering; Diseases; Genomics; Humans; Instruments; Microorganisms; Pathogens; Polymers; Viruses (medical);

fLanguage

English

Journal_Title

Proceedings of the IEEE

Publisher

ieee

ISSN

0018-9219

Type

jour

DOI

10.1109/JPROC.2002.804680

Filename

1046951