Title :
Biological cell alignment for electrofusion
Author :
Macdonald, S.J. ; Bodger, P.S. ; Elder, P.A.
Author_Institution :
Dept. of Electr. & Electron. Eng., Canterbury Univ., Christchurch, New Zealand
fDate :
5/1/1992 12:00:00 AM
Abstract :
The alignment of biological cells by dielectrophoresis was investigated as a preliminary to electrofusion of dissimilar cells. An output buffer circuit was built to couple a commercial signal generator to a low-impedance load. The system was used to apply an alternating electric field to a solution of white blood and mouse myeloma cells to align these into close membrane contact. Electrofusion of these cells was successful.
Keywords :
bioelectric phenomena; biological effects of fields; blood; cellular biophysics; electrophoresis; signal generators; biological cell alignment; close membrane contact; dielectrophoresis; dissimilar cell electrofusion; low-impedance load; mouse myeloma cells; output buffer circuit; signal generator; white blood cells;
Journal_Title :
Science, Measurement and Technology, IEE Proceedings A
