Measurement of acetylcholine receptor function in microcircuit-coupled myoblasts

Author

Quong, Judy N. ; Fare, Thomas L. ; Bryant, Howard J. ; Stenger, David A.

Author_Institution

Geo- Centers Inc., Ft. Washington, MD, USA

Volume

40

Issue

11

fYear

1993

Firstpage

1122

Lastpage

1126

Abstract

An electrophysiological measurement principle for long-term, noninvasive monitoring of the nicotinic acetylcholine receptor function is described. The measurement is based on the ability to record agonist-induced depolarizations of clonal myoblasts that have formed high impedance seals with extracellular microcircuit electrodes. The technique appears promising for several types of assays and environmental monitoring applications.

Keywords

bioelectric phenomena; biological techniques and instruments; cellular transport and dynamics; muscle; neurophysiology; agonist-induced depolarizations; assays; electrophysiological measurement principle; environmental monitoring; extracellular microcircuit electrodes; high impedance seals; long-term noninvasive monitoring; microcircuit-coupled myoblasts; neurotransmitter; nicotinic acetylcholine receptor function; Biomembranes; Cells (biology); Electrodes; Electrophysiology; Extracellular; Impedance; Impedance measurement; Microelectrodes; Monitoring; Proteins; Resists; Seals; Silicon compounds; Animals; Biosensing Techniques; Cell Membrane; Cells, Cultured; Electric Impedance; Electrophysiology; Mammals; Microelectrodes; Monitoring, Physiologic; Muscles; Receptors, Cholinergic;

fLanguage

English

Journal_Title

Biomedical Engineering, IEEE Transactions on

Publisher

ieee

ISSN

0018-9294

Type

jour

DOI

10.1109/10.245629

Filename

245629