Title :
Measurement of acetylcholine receptor function in microcircuit-coupled myoblasts
Author :
Quong, Judy N. ; Fare, Thomas L. ; Bryant, Howard J. ; Stenger, David A.
Author_Institution :
Geo- Centers Inc., Ft. Washington, MD, USA
Abstract :
An electrophysiological measurement principle for long-term, noninvasive monitoring of the nicotinic acetylcholine receptor function is described. The measurement is based on the ability to record agonist-induced depolarizations of clonal myoblasts that have formed high impedance seals with extracellular microcircuit electrodes. The technique appears promising for several types of assays and environmental monitoring applications.
Keywords :
bioelectric phenomena; biological techniques and instruments; cellular transport and dynamics; muscle; neurophysiology; agonist-induced depolarizations; assays; electrophysiological measurement principle; environmental monitoring; extracellular microcircuit electrodes; high impedance seals; long-term noninvasive monitoring; microcircuit-coupled myoblasts; neurotransmitter; nicotinic acetylcholine receptor function; Biomembranes; Cells (biology); Electrodes; Electrophysiology; Extracellular; Impedance; Impedance measurement; Microelectrodes; Monitoring; Proteins; Resists; Seals; Silicon compounds; Animals; Biosensing Techniques; Cell Membrane; Cells, Cultured; Electric Impedance; Electrophysiology; Mammals; Microelectrodes; Monitoring, Physiologic; Muscles; Receptors, Cholinergic;
Journal_Title :
Biomedical Engineering, IEEE Transactions on
