Title :
Plasmonic Sensing of Biological Analytes Through Nanoholes
Author :
Hwang, Grace M. ; Pang, Lin ; Mullen, Elaine H. ; Fainman, Yeshaiahu
Author_Institution :
MITRE Corp., Bedford, MA
Abstract :
A transmission-based surface plasmon resonance (SPR) sensor for label-free detection of protein-carbohydrate and protein-protein binding proximate to a perforated gold surface is demonstrated. An SPR instrument makes real-time measurements of the resonant wavelength and/or the resonant angle of incidence of transmitted light; both are influenced by the presence of proteins at the gold surface-liquid interface. Ethylene glycol solutions with known refractive indexes were used to calibrate the instrument. A paired polarization-sensitive detector achieved an overall detection resolution of ~ 6.6 times 10-5 refractive index units (RIU). Proof of principle experiments was performed with concanavalin A (Con A) binding to gold-adsorbed ovomucoid and anti-bovine serum albumin (BSA) binding to gold-adsorbed BSA.
Keywords :
biosensors; chemical sensors; gold; macromolecules; molecular biophysics; nanostructured materials; polaritons; proteins; surface plasmon resonance; anti-bovine serum albumin; biological analytes; concanavalin A binding; ethylene glycol solution; gold nanohole arrays; gold surface-liquid interface; label-free detection; ovomucoid; paired polarization-sensitive detector; perforated gold surface; protein-carbohydrate binding; protein-protein binding; refractive index; transmission-based surface plasmon resonance sensor; Biosensors; Gold; Instruments; Nanobioscience; Plasmons; Proteins; Refractive index; Resonance; Surface waves; Wavelength measurement; Bioplasmonics; biosensors; gold nanohole array; surface plasmon polariton (SPP); surface plasmon resonance (SPR);
Journal_Title :
Sensors Journal, IEEE
DOI :
10.1109/JSEN.2008.2007663
