بيان نسبي ژنهاي كليدي متابوليسم ليپيد بهدنبال مصرف رژيم غذايي پرچرب و تمرين هوازي در كبد موش صحرايي

Relative Gene Expression of Key Genes Involved in Lipid Metabolism, Following High Fat Diet and Moderate and High Intensity Aerobic Training in Rat’s Liver

ﺑﻪﻣﻨﻈﻮر ﺑﺮرﺳﯽ ﺳﻮﺧﺖوﺳﺎز ﮐﺒﺪي ﻟﯿﭙﯿﺪﻫﺎ، 44 ﺳﺮ ﻣﻮش ﺻﺤﺮاﯾﯽ ﻧﺮ ﻧﮋاد وﯾﺴﺘﺎر ﺑﻪﻃﻮر ﺗﺼﺎدﻓﯽ در دو ﮔﺮوه ﺑﺎ رژﯾﻢ ﻏﺬاﯾﯽ ﻃﺒﯿﻌﯽ و ﭘﺮﭼﺮب ﻗﺮار ﮔﺮﻓﺘﻨﺪ. ﻫﺮ ﮔﺮوه ﺑﻪ ﺳﻪ زﯾﺮﮔﺮوه ﮐﻨﺘﺮل، ﮔﺮوه ﺑﺎ ﺗﻤﺮﯾﻦ ﻫﻮازي ﻣﺘﻮﺳﻂ و ﮔﺮوه ﺑﺎ ﺗﻤﺮﯾﻦ ﻫﻮازي ﺷﺪﯾﺪ ﺗﻘﺴﯿﻢ ﺷﺪ. ﭘﺲ از ﻫﺸﺖ ﻫﻔﺘﻪ ﺗﻤﺮﯾﻦ، ﺑﯿﺎن ﻧﺴﺒﯽ ژنﻫﺎي ﮔﯿﺮﻧﺪة X ﻓﺎرﻧﺰوﯾﺪ (FXR)، ﮔﯿﺮﻧﺪة ﻓﻌﺎلﺷﺪه ﺑﺎ ﺗﮑﺜﯿﺮ ﭘﺮواﮐﺴﯿﺰوم آﻟﻔﺎ PPAR-α و ﭘﺮوﺗﺌﯿﻦ ﭘﯿﻮﻧﺪي ﻋﻨﺼﺮ ﺗﻨﻈﯿﻤﯽ اﺳﺘﺮول (1c SREBP-1c) در ﺑﺎﻓﺖ ﮐﺒﺪ ﻣﻮرد ارزﯾﺎﺑﯽ ﻗﺮار ﮔﺮﻓﺖ. ﻫﻤﭽﻨﯿﻦ، ﻣﻘﺎدﯾﺮ ﭘﻼﺳﻤﺎﯾﯽ ﻧﯿﻢرخ ﻟﯿﭙﯿﺪ و آﻧﺰﯾﻢﻫﺎي آﻣﯿﻨﻮﺗﺮﻧﺴﻔﺮاز ﺑﺮاي ﻣﻄﺎﻟﻌﺎت ﺑﯿﺸﺘﺮ اﻧﺪازهﮔﯿﺮي ﺷﺪ. دادهﻫﺎ ﻧﯿﺰ ﺗﻮﺳﻂ آزﻣﻮن ﺗﺤﻠﯿﻞ وارﯾﺎﻧﺲ دوﺳﻮﯾﻪ و در ﻧﺮماﻓﺰار اس.ﭘﯽ.اس.اس ﻧﺴﺨﮥ 22، در ﺳﻄﺢ ﻣﻌﻨﺎداري 95 درﺻﺪ ﺗﺠﺰﯾﻪوﺗﺤﻠﯿﻞ ﮔﺮدﯾﺪ. ﺑﺮﻣﺒﻨﺎي ﻧﺘﺎﯾﺞ، رژﯾﻢ ﻏﺬاﯾﯽ ﭘﺮﭼﺮب ﺑﺎﻋﺚ اﺧﺘﻼل ﻟﯿﭙﯿﺪ و اﺣﺘﻤﺎﻻً آﺳﯿﺐ ﮐﺒﺪي ﺷﺪه و ﺗﻨﻬﺎ ﺑﯿﺎن ژن SREBP-1c را اﻓﺰاﯾﺶ داده اﺳﺖ. ﻫﻤﭽﻨﯿﻦ، ﺑﺎوﺟﻮداﯾﻦﮐﻪ ﺗﻤﺮﯾﻦ اﺛﺮي ﺑﺮ ﺑﯿﺎن ژن FXR ﻧﺪاﺷﺘﻪ اﺳﺖ؛ اﻣﺎ ﺑﯿﺎن PPAR-α ﻣﺴﺘﻘﻞ از ﺷﺪت ﺗﻤﺮﯾﻦ در ﮔﺮوهﻫﺎي ﺑﺎ رژﯾﻢ ﻏﺬاي ﭘﺮﭼﺮب را اﻓﺰاﯾﺶ داده اﺳﺖ. ﻧﺘﺎﯾﺞ ﺣﺎﮐﯽ از آن ﻫﺴﺘﻨﺪ ﮐﻪ ﺑﯿﺎن SREBP-1c در ﮔﺮوه ﺑﺎ ﺗﻐﺬﯾﮥ ﻃﺒﯿﻌﯽ ﺑﺎ ﺗﻤﺮﯾﻦ ﺷﺪﯾﺪ ﮐﺎﻫﺶ ﭘﯿﺪا ﮐﺮده اﺳﺖ. ﺑﻪﻃﻮرﮐﻠﯽ، اﺧﺘﻼل ﻟﯿﭙﯿﺪ و ﺗﺠﻤﻊ ﭼﺮﺑﯽ در ﺑﺎﻓﺖ ﮐﺒﺪ ﻣﯽﺗﻮاﻧﺪ از ﻋﻮاﻗﺐ ﻣﺼﺮف رژﯾﻢﻫﺎي ﭘﺮﭼﺮب ﺑﺎﺷﺪ ﮐﻪ اﺣﺘﻤﺎﻻً از ﻃﺮﯾﻖ اﻓﺰاﯾﺶ ﺑﯿﺎن SREBP-1c ﮐﻪ ﻣﻨﺠﺮ ﺑﻪ ﻓﻌﺎلﺷﺪن ﻣﺴﯿﺮﻫﺎي ﻟﯿﭙﻮژﻧﯿﮏ ﻣﯽﺷﻮد، ﺻﻮرت ﻣﯽﮔﯿﺮد؛ اﮔﺮﭼﻪ ﻣﺪاﺧﻼت ﺗﻤﺮﯾﻨﯽ اﯾﻦ ﭘﮋوﻫﺶ ﺑﯿﺎن ژنﻫﺎ را ﺗﻐﯿﯿﺮ داد؛ اﻣﺎ اﺛﺮ ﻣﺸﻬﻮدي ﺑﺮ ﭘﺮوﻓﺎﯾﻞ ﻟﯿﭙﯿﺪ و آﻣﯿﻨﻮﺗﺮﻧﺴﻔﺮازﻫﺎ ﻧﺪاﺷﺖ.

To study the hepatic lipid metabolism, 44 male Wistar rats randomly divided in two normal or high fat diet groups. Each group was included of control, moderate exercise and high intensity exercise groups. After the 8 weeks of training hepatic relative gene expression of Farnesoid X Receptor (FXR), Peroxisome proliferator activated receptor alpha (PPAR-α) and also sterol regulatory element binding protein 1c (SREBP-1c) were assessed. Also, plasma lipid profile and Aminotransferases levels were measured. Data were analyzed by 2way ANOVA in SPSS 22 (P<0.05). According to the results, high fat diet caused dyslipidemia and probably liver injury and only increased SERBP-1crelative gene expression. Although training had not any effect on FXR expression, PPAR-α expression increased in trained high fat diet groups, independent to intensity. SREBP-1c expression just decreased by high intensity training in normal diet group. In summary, dyslipidemia and hepatic fat overload could be induced by high fat diet due to hepatic SREBP-1c expression and activation of lipogenic pathways. Although exercise changed the gene expression but had no effect on lipid profile or transaminases.