ﺗﻐﯿﯿﺮات ﺳﺎﯾﺘﻮﮐﺎﯾﻦﻫﺎي اﯾﻨﺘﺮﻟﻮﮐﯿﻦ ـ2 و اﯾﻨﺘﺮﻟﻮﮐﯿﻦ ـ12 در ﺳﻠﻮلﻫﺎي ﻃﺤﺎل ﻣﻮشﻫﺎي ﺑﺎﻟﺐﺳﯽ ﺑﻪدﻧﺒﺎل اﺳﺘﻔﺎده از ﯾﮏ وﻫﻠﻪ ورزش اﺳﺘﻘﺎﻣﺘﯽ ﺑﻪﻋﻨﻮان اﺟﻮاﻧﺖ واﮐﺴﻦ وﯾﺮوس ﻫﺮﭘﺲ ﺳﯿﻤﭙﻠﮑﺲ ﯾﮏ

Changes in Interleukin-2 and Interleukin-12 Cytokines in Spleen Cells of Balb-C Mice After Using an Acute Endurance Exercise as An Adjuvant for Herpes Simplex Virus 1 Vaccine

در راﺳﺘﺎي اﻓﺰاﯾﺶ ﮐﺎراﯾﯽ واﮐﺴﻦﻫﺎ، اﺳﺘﻔﺎده از اﺟﻮاﻧﺖﻫﺎ ﻫﻤﻮاره ﻣﻮردﺗﻮﺟﻪ اﯾﻤﻮﻧﻮﻟﻮژﯾﺴﺖﻫﺎ ﺑﻮده اﺳﺖ. ﻓﻌﺎﻟﯿﺖﻫﺎي ورزﺷﯽ ﺑﺎ ﺗﻮﺟﻪ ﺑﻪ ﺗﻐﯿﯿﺮات اﯾﻤﻮﻧﻮﻟﻮژﯾﮑﯽ ﮐﻪ اﯾﺠﺎد ﻣﯽﮐﻨﻨﺪ ﻣﯽﺗﻮاﻧﻨﺪ ﺑﻪﻋﻨﻮان اﺟﻮاﻧﺖ واﮐﺴﻦ ﺑﺮاي ﭘﺎﺳﺦﻫﺎي اﯾﻤﻨﯽ ﻣﺆﺛﺮ ﺑﺎﺷﻨﺪ. در اﯾﻦ ارﺗﺒﺎط، ﻫﺪف از ﭘﮋوﻫﺶ ﺣﺎﺿﺮ ﺑﺮرﺳﯽ اﺛﺮ اﺟﻮاﻧﺘﯽ ﯾﮏ وﻫﻠﻪ ورزش اﺳﺘﻘﺎﻣﺘﯽ ﺑﺮ ﺗﻮﻟﯿﺪ ﺳﺎﯾﺘﻮﮐﺎﯾﻦﻫﺎي اﯾﻨﺘﺮﻟﻮﮐﯿﻦ ـ2 و اﯾﻨﺘﺮﻟﻮﮐﯿﻦ ـ12 از ﺳﻠﻮلﻫﺎي ﻃﺤﺎل در ﻣﺪل واﮐﺴﻦ وﯾﺮوس ﻫﺮﭘﺲ ﺳﯿﻤﭙﻠﮑﺲ ﯾﮏ ﺑﻮد. ﺑﺪﯾﻦﻣﻨﻈﻮر، 24 ﻣﻮش ﻧﮋاد ﺑﺎﻟﺐﺳﯽ 5ـ4 ﻫﻔﺘﻪ )ﺑﺎ ﻣﯿﺎﻧﮕﯿﻦ وزﻧﯽ 17/67 ﮔﺮم( ﺑﻪ ﺳﻪ ﮔﺮوه ﮐﻨﺘﺮل، ﮔﺮوه واﮐﺴﻦ و درﯾﺎﻓﺖﮐﻨﻨﺪة واﮐﺴﻦ و ﯾﮏ وﻫﻠﻪ ورزش اﺳﺘﻘﺎﻣﺘﯽ ﺗﻘﺴﯿﻢ ﮔﺮدﯾﺪﻧﺪ. دو ﻫﻔﺘﻪ ﺑﻌﺪ از ﺳﻪ دوره واﮐﺴﻦ، ﺗﻮﻟﯿﺪ ﺳﺎﯾﺘﻮﮐﺎﯾﻦﻫﺎي اﯾﻨﺘﺮﻟﻮﮐﯿﻦ ـ2 و اﯾﻨﺘﺮﻟﻮﮐﯿﻦ ـ12 ﭘﺲ از ﮐﺸﺖ ﺳﻠﻮﻟﯽ ﻃﺤﺎل ﺗﻮﺳﻂ ﺗﮑﻨﯿﮏ اﻻﯾﺰا ﺳﻨﺠﯿﺪه ﺷﺪ. ﺑﺮاي ﺑﺮرﺳﯽ ﻣﺘﻐﯿﺮﻫﺎي ﭘﮋوﻫﺶ از آﻧﺎﻟﯿﺰ وارﯾﺎﻧﺲ ﯾﮏﻃﺮﻓﻪ اﺳﺘﻔﺎده ﮔﺮدﯾﺪ. ﻧﺘﺎﯾﺞ ﻧﺸﺎن ﻣﯽدﻫﺪ ﮐﻪ ﺑﯿﻦ ﮔﺮوهﻫﺎي ﭘﮋوﻫﺶ درﻣﻮرد ﺳﺎﯾﺘﻮﮐﺎﯾﻦ اﯾﻨﺘﺮﻟﻮﮐﯿﻦ ـ2 ﺗﻔﺎوت ﻣﻌﻨﺎداري وﺟﻮد دارد (P=0.008) ﻧﺘﺎﯾﺞ آزﻣﻮن ﭘﯿﮕﯿﺮي ﺗﻮﮐﯽ ﻧﯿﺰ ﻧﺸﺎندﻫﻨﺪة وﺟﻮد ﺗﻔﺎوت ﻣﻌﻨﺎدار ﺑﯿﻦ ﮔﺮوهﻫﺎي ﮐﻨﺘﺮل و درﯾﺎﻓﺖﮐﻨﻨﺪة واﮐﺴﻦ و ﯾﮏ وﻫﻠﻪ ورزش اﺳﺘﻘﺎﻣﺘﯽ ﻣﯽﺑﺎﺷﺪ (P=0.006)؛ اﻣﺎ ﺑﺎ وﺟﻮد اﻓﺰاﯾﺶ ﺗﻮﻟﯿﺪ ﺳﺎﯾﺘﻮﮐﺎﯾﻦ اﯾﻨﺘﺮﻟﻮﮐﯿﻦ ـ12 در ﮔﺮوه واﮐﺴﻦ و ﯾﮏ وﻫﻠﻪ ورزش اﺳﺘﻘﺎﻣﺘﯽ، اﯾﻦ اﻓﺰاﯾﺶ ﻣﻌﻨﺎدار ﻧﻤﯽﺑﺎﺷﺪ. ﻫﻤﭽﻨﯿﻦ، ﻧﺘﺎﯾﺞ ﺣﺎﮐﯽ از اﻓﺰاﯾﺶ ﺗﻮﻟﯿﺪ ﺳﺎﯾﺘﻮﮐﺎﯾﻦ اﯾﻨﺘﺮﻟﻮﮐﯿﻦ ـ2 از ﺳﻠﻮلﻫﺎي ﻃﺤﺎل ﺑﻪدﻧﺒﺎل اﺳﺘﻔﺎده از ﯾﮏ وﻫﻠﻪ ورزش اﺳﺘﻘﺎﻣﺘﯽ ﺑﻪﻋﻨﻮان اﺟﻮاﻧﺖ اﺳﺖ. ﺑﻪﻧﻈﺮ ﻣﯽرﺳﺪ ﮐﻪ اﺳﺘﻔﺎده از ﯾﮏ وﻫﻠﻪ ورزش اﺳﺘﻘﺎﻣﺘﯽ ﺑﻪﻋﻨﻮان اﺟﻮاﻧﺖ واﮐﺴﻦ وﯾﺮوس ﻫﺮﭘﺲ ﺳﯿﻤﭙﻠﮑﺲ ﯾﮏ اﺣﺘﻤﺎﻻً ﺗﻮاﻧﺴﺘﻪ اﺳﺖ ﮐﺎراﯾﯽ ﺳﯿﺴﺘﻢ اﯾﻤﻨﯽ را درﺟﻬﺖ ﺳﻠﻮلﻫﺎي ﮐﻤﮑﯽ ﻧﻮع ﯾﮏ ﺑﻪﻣﻨﻈﻮر ﻣﺒﺎرزه ﺑﺎ ﻋﻔﻮﻧﺖﻫﺎي وﯾﺮوﺳﯽ ﺗﻘﻮﯾﺖ ﮐﻨﺪ.

For vaccine efficacy always using adjuvant has been considered by immunologists. Exercise can induce immune changes, and therefore, has been identified as an adjuvant for immune responses. The purpose of this study was to assess adjuvant effects of an acute endurance exercise on interleukin-2 and interleukin-12 cytokines of spleen cells in herpes simplex virus 1 vaccine model. 24 BALB/c mice, 4 to 5 weeks old and with an average weight of 17.6 g, were divided into 3 groups: control, vaccine and vaccine plus an acute endurance exercise. Two weeks after three booster shots of vaccine, interleukin-2 and interleukin-12 levels was determined in spleen cell culture with ELISA method. One-ways ANOVA statistical test were used for data analysis. The result of this study indicated that there are significant differences between groups in interleukin-2 (P=0.008). Tukey post hoc test results showed significant differences between control and vaccine plus an acute endurance exercise groups (P=0.006). Changes in interleukin-12 cytokine production in vaccine and endurance exercise group were not significant. Result of current study showed that interleukin-2 levels from spleen cells increased following using an acute endurance exercise as an adjuvant. It seems that using an acute endurance exercise probably increased efficacy of immune system in direction to T helper 1 cells against virus infections.