ﺗﺄﺛﯿﺮ ﺗﻤﺮﯾﻦ ﺗﻨﺎوﺑﯽ ﺷﺪﯾﺪ و ﻣﮑﻤﻞ ﺳﺎزي روﻏﻦ ﺑﺬر ﮐﺘﺎن ﺑﺮ اﺣﺴﺎس درد اﻟﻘﺎﺷﺪه در ﻣﻮشﻫﺎي ﺻﺤﺮاﯾﯽ ﻧﺮ

The Effects of High Intensity Interval Training and Flaxseed Oil Supplement on Pain Feeling in Male Rats

ﻫﺪف از ﭘﮋوﻫﺶ ﺣﺎﺿﺮ، ﺑﺮرﺳﯽ ﺗﺄﺛﯿﺮ ﯾﮏ دوره ﺗﻤﺮﯾﻦ ﺗﻨﺎوﺑﯽ ﺷﺪﯾﺪ و ﻣﮑﻤﻞﺳﺎزي روﻏﻦ ﺑﺬر ﮐﺘﺎن ﺑﺮ درد اﻟﻘﺎﺷﺪه در ﻣﻮشﻫﺎي ﺻﺤﺮاﯾﯽ ﻧﺮ ﺑﻮد. ﺑﺪﯾﻦﻣﻨﻈﻮر، 40 ﺳﺮ ﻣﻮش ﺻﺤﺮاﯾﯽ ﺑﺎﻟﻎ از ﻧﮋاد وﯾﺴﺘﺎر ﺑﻪﻋﻨﻮان ﻧﻤﻮﻧﮥ آﻣﺎري اﻧﺘﺨﺎب ﺷﺪه و ﺑﻪﺻﻮرت ﺗﺼﺎدﻓﯽ ﺑﻪ ﻫﺸﺖ ﮔﺮوه )ﭘﻨﺞ ﺳﺮ در ﻫﺮ ﮔﺮوه( ﮐﻨﺘﺮل ـ ﺳﺎﻟﯿﻦ، ﺗﻤﺮﯾﻦ ـ ﺳﺎﻟﯿﻦ، ﻋﺼﺎرة دوز 100، ﻋﺼﺎرة دوز 200 ﻋﺼﺎرة دوز 300، ﺗﻤﺮﯾﻦ ـ ﻋﺼﺎرة دوز 100، ﺗﻤﺮﯾﻦ ـ ﻋﺼﺎرة دوز 200 و ﺗﻤﺮﯾﻦ ـ ﻋﺼﺎرة دوز 300 ﻣﯿﻠﯽﮔﺮم ﺑﺮ ﮐﯿﻠﻮﮔﺮم ﺗﻘﺴﯿﻢ ﮔﺮدﯾﺪﻧﺪ. ﮔﺮوهﻫﺎي ﺗﻤﺮﯾﻨﯽ ﺑﺎ 90 ﺗﺎ 95 درﺻﺪ ﺣﺪاﮐﺜﺮ اﮐﺴﯿﮋن ﻣﺼﺮﻓﯽ ﺑﻪ ﺗﻤﺮﯾﻦ اﯾﻨﺘﺮوال روي ﻧﻮارﮔﺮدان ﻣﺨﺼﻮص ﺟﻮﻧﺪﮔﺎن ﭘﺮداﺧﺘﻨﺪ )10 ﻫﻔﺘﻪ، ﭘﻨﺞ ﺟﻠﺴﻪ در ﻫﻔﺘﻪ( و ﮔﺮوهﻫﺎي ﻣﮑﻤﻞ، روﻏﻦ ﺑﺬر ﮐﺘﺎن را درﯾﺎﻓﺖ ﻧﻤﻮدﻧﺪ. ارزﯾﺎﺑﯽ آﺳﺘﺎﻧﮥ اﺣﺴﺎس درد ﺗﻮﺳﻂ آزﻣﻮن ﺻﻔﺤﮥ داغ ﺑﺎ دﻣﺎي 55±0/5 درﺟﮥ ﺳﺎﻧﺘﯽﮔﺮاد ﺻﻮرت ﮔﺮﻓﺖ. ﺑﺮاي ﺑﺮرﺳﯽ ﺗﻔﺎوت ﺑﯿﻦ ﮔﺮوهﻫﺎ ﻧﯿﺰ از آزﻣﻮن آﻧﺎﻟﯿﺰ وارﯾﺎﻧﺲ دوﻃﺮﻓﻪ اﺳﺘﻔﺎده ﺷﺪ و ﺳﻄﺢ ﻣﻌﻨﺎداري ﮐﻤﺘﺮ از (0/05) ﻟﺤﺎظ ﮔﺮدﯾﺪ. ﻧﺘﺎﯾﺞ ﻧﺸﺎن ﻣﯽدﻫﺪ ﮐﻪ اﺳﺘﻔﺎده از ﻣﮑﻤﻞ ﺑﺎﻋﺚ ﮐﺎﻫﺶ ﻣﻌﻨﺎدار اﺣﺴﺎس درد ﺷﺪه اﺳﺖ (0.004=P) ﮐﻪ اﻟﺒﺘﻪ اﯾﻦ ﮐﺎﻫﺶ در ﮔﺮوه ﻋﺼﺎره دوز 300 ﺑﻪﺷﮑﻞ ﻣﻌﻨﺎداري ﺑﺎﻻﺗﺮ از ﮔﺮوه ﻋﺼﺎره دوز 100 اﺳﺖ (P=0.01). ﻫﻤﭽﻨﯿﻦ، اﺣﺴﺎس درد در ﮔﺮوهﻫﺎي ﺗﻌﺎﻣﻠﯽ ﺗﻤﺮﯾﻦ ﻣﮑﻤﻞ ﺑﻪﻃﻮر ﻣﻌﻨﺎداري ﮐﻤﺘﺮ از ﮔﺮوهﻫﺎي ﺗﻤﺮﯾﻦ و ﮐﻨﺘﺮل ـ ﺳﺎﻟﯿﻦ ﻣﯽﺑﺎﺷﺪ (P<0.05). اﺣﺘﻤﺎﻻ ً ﻣﺼﺮف ﻣﮑﻤﻞ ﺑﻪدﻟﯿﻞ وﺟﻮد وﯾﺘﺎﻣﯿﻦﻫﺎ و روﻏﻦﻫﺎي ﻏﯿﺮاﺷﺒﺎع ﻣﻔﯿﺪ ﻣﯽﺗﻮاﻧﺪ اﺣﺴﺎس درد ﻧﺎﺷﯽ از ﺗﻤﺮﯾﻦ ﺷﺪﯾﺪ را ﺗﻌﺪﯾﻞ ﮐﻨﺪ؛ ﺑﻨﺎﺑﺮاﯾﻦ، اﺳﺘﻔﺎده از روﻏﻦ ﺑﺬر ﮐﺘﺎن ﺟﻬﺖ ﮐﺎﻫﺶ درد ﻧﺎﺷﯽ از ﻓﻌﺎﻟﯿﺖ ﺑﺪﻧﯽ ﺳﻨﮕﯿﻦ ﺗﻮﺻﯿﻪ ﻣﯽﮔﺮدد.

This study investigates the effect of high intensity interval training and flaxseed oil supplement on pain feeling in male rats. Forty adult Wistar rats were randomly divided into eight groups (five in each group) including control- saline, training- saline, extracts dose of 100 mg/kg, extracts dose of 200 mg/kg, extracts dose of 300 mg/kg, extracts dose of 100 mg/kg- training, extracts dose of 200 mg/kg-training and extracts dose of 300 mg/kg-training. Training groups was given high-intensity interval training (10 weeks, five sessions in week) on a rodent treadmill at 90 to 95 percent of VO2max and supplement groups also received flaxseed oil. Pain threshold assessment by the hot plate test with a temperature of 55 ± 0.5° C. Statistical analyses were performed using a two-way analysis of variance. Least significant difference posts hoc test was used in the event of a significant (P<0.05). Result showed that supplement induced significant decreases in pain feeling (P=0.004) that extracts dose of 300 group has lower pain feeling than extracts dose of 100 (P=0.012). Pain feeling significantly lower in the group of supplements-exercises than exercise and control-saline groups (P<0.05). Flaxseed oil due to vitamins and unsaturated fatty acids has been able to reduce the pain of intense exercise. Therefore, flaxseed oil supplement to reduce pain of heavy physical activity is recommended.