تاثير اسپرم گيري مجدد بر برخي از شاخص هاي كيفي اسپرم ماهي سفيدك سيستان (Schizothorax zarudnyi Nikolskii، 1897 )

Effect of repetitive sperm stripping on some sperm quality indicators of Schizothorax zarudnyi

The survey was carried out at the Zahak reproduction center on 11 mature males of Schizothorax zarudnyi at 2013. Each male، was injected by Oviprime hormone 3 times in 7 day interval. Sperm was stripped after each injection but no sperm was not obtained at the third stage. Spermatology parameters were measured in the first and second stage. The results showed that average duration of sperm motility was (45.09 ± 7.73) in the first stage of stripped sperm sampling and (52.84 ±5.20) in the second stage that the difference was not significant (p>0.05). The percentage of motile spermatozoa was (57.18 ±11.29) and (72.4 ±9.73) in the first and second stage respectively that the difference was not significant (p>0.05). According to the results of this study، the volume of sperm was higher in the first stage of sampling compared with the second one that this difference was not significant (p>0.05). The average of sperm density was higher in the second stage (0.8±0.10) compared with the first one (0.7±0.84) that the difference was not significant (p>0.05). The percentage of spermatocrit was obtained (35 ±7.16 and 33.55 ±2.91) and pH was (8.03 ± 0.12 and 7.78 ± 0.27) in the first and second stages respectively that there is a significant difference (p<0.05). The results showed that adjusting appropriate intervals for repetitive sperm stripping depending on the fish species in hatchery can be an effective technique in reproduction centers.