استفاده از آب پنير بعنوان محيط كشت جايگزين در توليد جلبك دوناليلا سالينا

Use of cheese whey as an alternative culture medium for the production of Donalia Salina algae

هدف اين تحقيق بررسي اثر غلظت‌هاي مختلف آب پنير و محيط كشت والنه جهت توليد بيومس Dunaliella salina و ارزيابي خصوصيات بيوشيميايي آن بود. بدين منظور سه فاكتور شامل غلظت محيط كشت والنه (صفر تا 50 ميكروليتر)، درصد آب پنير (صفر تا 5 درصد) و زمان گرمخانه گذاري (صفر تا 14 روز) در قالب طرح باكس بانكن شامل 17 نمونه مورد مطالعه قرار گرفت. نتايج نشان داد با افزايش درصد آب پنير و همچنين محيط كشت والنه، تراكم سلولي افزايش يافت ولي اين افزايش از نظر آماري معني‌دار نبود (0/05

The aim of this study was to investigate the effects of different concentrations of cheese whey and Walne culture medium to produce Dunaliella salina biomass and evaluation of its biochemical properties. For this purpose, three factors including the concentration of Walne culture medium (0-50 μl), the percentage of cheese whey (0-5 %) and incubation time (0-14 days) using Box-Behnken design with 17 Runs were studied. Results showed that increasing of cheese whey percentage and Walne culture medium caused to increase cell density but it was not statistically significant (p>0.05). Increasing of the Walne culture medium led to increasing chlorophyll and carotenoids and showed a significant difference (p<0.05). The cell densityand the amount of chlorophyll and carotenoids decreased gradually with increasing incubation time, however, there was no significant difference observed in the levels of chlorophyll and carotenoids (p>0.05). Significant differences were observed between the different treatments in producing cell density (p<0.05). The protein content was significantly increased with increasing of cheese whey percentage and incubation period up to 7th (p<0.05). Also, increasing in Walne culture medium concentration resulted in increasing the protein content until day 7 of the incubation period and decreased (from day 9 onwards) over time and reached its lowest level on day 14 but no significant differences was detected through the period (p>0.05). Optimum conditions were obtained on day 5 of incubation, 5 percent of cheese whey and zero percent of the Walne culture medium. In the mentioned condition the amount of cell density, protei and carotenoid was 2.74⨯10+7, 288.75 mg/kg, 0.202 mg/ml respectively.