نقش گياه پنج انگشت (Vitex agnus-castus) دركنترل روند رسيدگي جنسي ماهي قرمز (Carrasius auratus)

The role‌‌ of different concentration of Vitex agnus-castus on control of ripeness of gonad of Carassius auratus

امروزه تاثير فيتو استروژن­ ها بر روند طيبعي هورمون­هاي جنسي در ماهيان بعنوان يك عامل تاثير­گذار به اثبات رسيده و هدف اين پژوهش بررسي تاثير عصاره گياه پنج انگشت (Vitex agnus-castus) در اين روند بوده است. در اين راستا اثر اين گياه بر ساختار گناد ماهي قرمز (Carasius auratus) مورد بررسي قرار گرفت. بدين منظور 180 عدد ماهي ماده با ميانگين وزني 0/8±16/5گرم انتخاب شدند. سپس ماهيان به مدت 48 ساعت با محيط عادت دهي شدند. فاكتورهاي فيزيكي و شيميايي آب نظير pH (6/5)، درجه حرارت (26) و اكسيژن محلول (6/5) تنظيم و آنگاه آزمايشات در 8 گروه (2 گروه كنترل و 4 گروه تيمار) با 3 تكرار كه هر گروه شامل 15 عدد ماهي ماده انجام شد. دوزهاي مختلف از عصاره گياه پنج انگشت (10، 20، 30 و 50 ميلي گرم بر هر كيلوگرم) بصورت عضلاني (IM) و به مقدار ml 0/2 در 10 نوبت طي 20 روز تزريق شد. تزريق­ها بمدت 20 روز صورت پذيرفت. در روز بيستم ماهيان بي­هوش گرديده و بيومتري ماهيان و اندازه گيري طول و وزن انجام شد. با انجام خونگيري از ساقه دمي مقدار هورمونهاي جنسي تستوسترون و 17 بتا استراديول به روش راديو ايمنواسي تعيين گرديد. ميزان هورمون 17 بتا استراديول در كليه تيمارهاي دريافت كننده عصاره گياه پنج انگشت، 10، 20، 30، 50 نسبت به گروه شاهد كاهش داشته كه كاملاً وابسته به دوز بود (P<0.01). تستوسترون نيز در تيمار 30 و 50 ميليگرم كاهش معني دار داشت لكن در تيمارهاي پايين تر نسبت به شاهد اختلاف معني­ دار نشان نداد. همچنين ميزان شاخص گنادوسوماتيك در تيمار 30 و 50 ميلي­ گرم كاهش يافته لكن قطر تخمك در اين دو تيمار اختلاف معني دار از خود نشان نداد. گناد ماهيان خارج و پس از انجام مراحل پاساژ بافت و رنگ آميزي با روش H&E در زير ميكروسكوپ بررسي شد. در مجموع عصاره گياه پنج انگشت در دوز­هاي 30 و 50 مانع از رشد و تكوين سلولهاي اوسيت در تمامي دوزها گرديد. اين عصاره مي تواند در روند كنترل توليد مثلي ماهيان تاثير داشته باشد.

Today, the influence of estrogen on the natural sex hormones in fish have been proven as a contributing factor.The effects of Vitexagnus-castus extracts were investigate on gonadal structure and reproductive performance of goldfish (C.auratus).Maturing females (n=180) with an average weight of 16/5 ± 0/8 g was stocked for 48 hours after adaptation to the environment. Physical and chemical factors such as pH(6.5), dissolved oxygen(6.5), temperature(26) adjusted to optimal condition. FToday, the influence of estrogen on the natural sex hormones in fish have been proven as a contributing factor. The effects of Vitex agnus-castus extracts were investigate on gonadal structure and reproductive performance of goldfish (C. auratus).Maturing females (n=180) with an average weight of 16/5 ± 0/8 g was stocked for 48 hours after adaptation to the environment. Physical and chemical factors such as pH (6.5), dissolved oxygen (6.5), temperature (26) adjusted to optimal condition. Gold Fishes were separated in 8 groups (2 control groups and 4 treatment groups) with three replications, each group contains 15 fishes. Different doses of extract (10, 20, 30 and 50 mg/kg) were injected intramuscularly (IM) for 20 days (10 times). Fishes were anesthetized after 21 days length and weight were measured. Gonads were taken for estimating gonadosomatic condition. Histological studies were considered by tissue processing and staining with H & E using light microscope. After blood sampling, Sex hormones (testosterone and 17 ß-estradiol) were determined by radioimmunoassay. 17- ß estradiol were decrease in all treatments copair to control groups and had dose dependent significant differences (P<0.01). Testosterone in 30 and 50 mg/kg treatments were also decreased significantly. Gonadosomatic index at 30 and 50 mg/kg treatments showed the same pattern witch supporting the inhibitory effects of Vitexagnus-castus extract on growth and development of oocyte and reproductive performance of fishes. ishes (Carrasius auratus) were separated in 8 groups (2 control groups and 4 treatment groups) with three replications, each group contains 15 fishes. Different doses of extract (10, 20, 30 and 50 mg/kg) were injected intramuscularly (IM) for 20 days(10 times). Fishes were anesthetized after 21 days theansfter length and weight were measured. Gonads were taken for estimating gonadosomatic condition. Histological studies were considered by tissue processing and staining with H & E using light microscope. After blood sampling, Sex hormones (testosterone and 17 ß-estradiol) were determined by radioimmunoassay. 17- ß estradiol were decrease in all treatments copair to control groups and had dose dependent significant differences (P<0.01). Testosterone in 30 and 50 mg/kg treatments were also decreased significantly.Gonadosomatic index at 30 and 50 mg/kg treatments showed the same patern witch supporting the inhibitory effects of Vitexagnus-castus exteract on growth and development of oocyte and reproductive performance of fishes.