تاثير تمرينات تناوبي پرشدت (HIIT) بر سطوح آيريزين و شاخص مقاومت انسولين در موش‌هاي صحرايي

EFFECT OF HIGH INTENSITY INTERVAL TRAINING (HIIT) ON LEVEL OF IRISIN and INSULIN RESISTANCE INDEX IN RATS

هدف: هدف از اجراي پژوهش، بررسي تاثير 10 هفته تمرين اينتروال پرشدت را بر سطوح سرمي آيريزين و شاخص مقاومت انسولين (HOMA-IR) در موش‌هاي صحرايي نر بود. روش شناسي: 16 سر موش صحرايي نر نژاد ويستار به طور تصادفي به دو گروه كنترل (8 سر) و تمريني (8 سر) تقسيم شدند. گروه تمريني، تمرينات اينتروال پرشدت (يك دقيقه دوي سرعت و 2-5 دقيقه استراحت، 6-10 بار در روز، 5-6 روز در هفته، با سرعت 30-80 متر بر دقيقه) را به مدت 10 هفته، روي تردميل اجرا كردند. 48 ساعت پس از آخرين جلسه تمريني نمونه‌هاي خوني جمع‌اوري و سطوح سرمي آيريزين و انسولين به روش الايزا و سطوح گلوكز به روش گلوكز اكسيداز اندازه‌گيري شدند. تجزيه و تحليل داده‌ها با آزمون آناليز واريانس يك سويه و آزمون همبستگي پيرسون در سطح معني‌داري 05/0>P انجام گرفت. نتايج: اجراي 10 هفته تمرينات تناوبي پرشدت افزايش معني دار سطوح سرمي آيريزين (004/0=P) را در پي داشت. علاوه بر اين در گروه تمرين سطوح گلوكز ناشتا و HOMA-IR كاهش يافت ولي اين كاهش معنادار نبود(05/0

Aims: The aim of this study was to investigate the effects of 10 week high intensity interval training on serum levels of irisin and insulin resistance index (HOMA-IR) in male rats. Methodology: sixteen male wistar rats were randomly divided in two control (n=8) and training (n=8) groups. Training group performed high intensity interval training (1 min/sprint, 6~10 sets/day and 5~6 days/week) for 10 weeks. Blood samples were collected 48 hours after last training session and serum levels of irisin, insulin and glucose were measured using ELISA and glucose oxidase method. Statistical analysis was done by ANOVA and Pearson correlation and (P<0.05) was considered significant. Results: serum levels of irisin (P=0.004) significantly increased following 10 week high intensity interval training. In addition serum levels of fasting glucose and HOMA-IR were decreased in the trained group but were not significant (P>0.05). Also serum levels of insulin in the trained group were more than control group but were not significant (P>0.05). Hence there were not significant correlations between irisin levels and insulin and HOMA-IR (P>0.05) but correlations between irisin and glucose were not significant (P=0.029). Conclusion: high intensity interval training increases irisin levels and improves insulin resistance index in male rats