مطالعه ايمونوهيستوشيميايي Expression نشانگر Ki-67 در آملوبلاستوماي تك حفره اي و كيست دانتي ژور

Immunohistochemicai study of Ki- 67 expression in unicystic Ameloblastoma and Dentigerous cyst

بيان مساله: تشخيص آملوبلاستوماي تك حفره اي از كيست دانتي ژور، كشف تغييرات آملوبلاستومايي ايجادشده در جدار كيست دانتي ژور در مراحل اوليه، تشخيص اپي تليوم ادنتوژن هيپرپلاستيك موجود در جدار همبندي كيست دانتي ژور از يك توده آملوبلاستومايي، همه مستلزم بكارگيري روشي دقيق و مطمئن است. هدف مطالعه حاضر با هدف بررسي و مقايسه واكنش ايمني شاخص تكثير سلولي Ki-67 در قسمتهاي مختلف اپي تليوم كيست دانتي ژور و آملوبلاستوماي تك حفره اي انجام شد. روش بررسي در اين مطالعه هم گروهي گذشته نگر، با استفاده از روش رنگ آميزي ايمونوهيستوشيمي، نشانگر Ki-67 بر روي 9 مورد آملوبلاستوماي تك حفره اي و 15 مورد كيست دانتي ژور، به كمك آنتي بادي MIB-1 رنگ شد. تعداد هسته هاي رنگ گرفته در دو ناحيه بازال و سوپرابازال اپي تليوم پوشاننده هر دو ضايعه، در 1000 سلول اپي تليالي شمارش و به عنوان Labeling Index (LI) بيان و مقايسه گرديد. داده هاي به دست آمده با استفاده از آزمون t-student تحليل گرديد. نتيجه گيري استفاده از نشانگر Ki-67 (LI) در ناحيه سوپرابازال يا كل ضخامت اپي تليوم پوشاننده براي مقايسه دو كيست، مي تواند به عنوان شاخصي براي تشخيص كيست دانتي ژور از آملوبلاستوماي تك حفره اي مورد استفاده قرار گيرد.

Statement of Problem: Differentiation of dentigerous cyst from unicystic ameloblastoma, discovering any initial ameloblastic changes in lining epithelium of dentigerous cyst at early stage, and differentiation between hyperplastic odontogenic epithelium in fibrous capsule of dentigerous cyst from ameloblastic proliferation, need to an accurate and reliable technique. Purpose The aim of this study was to determine and compare Ki-67 immunoreactivity in various locations of the epithelium of Dentigerous cyst and Unicystic Ameloblastoma. Materials an‎d Methods In this historical Cohort study, 15 cases of dentigerous cyst and 9 cases of unicystic ameloblastoma were selected. Immunohistochemistry staining was performed by M1B-1 (murine monoclonal antibody against Ki-67). The stained nucleous were counted in basal and suprabasal layer of lining epithelium of both lesions in 3000 epithelial cells. Finally, the percentage of positive cells (presented as labeling index) was calculated, t- student test was used to analyze the related data. Results Ki-67 (LI) in basal layer of Dentigerous cyst (2.59±1.66) and Unicystic Ameloblastoma (3.76±79) had no significant differences, but Ki-67 (LI) in suprabasal layer of unicystic ameloblastoma (2.15±0.69) was significantly higher than dentigerous cyst (0.77±0.55) P=0.003).The difference between the average numbers of positive cells for Ki-67 (LI) in these two lesions was statistically significant (P<0.05) and it was higher in Unicystic Ameloblastoma than Dentigerous cyst. Conclusion Based on the findings of this study, it is suggested that Ki-67 (LI) in suprabasal layer or throughout the epithelium can be considered as a useful marker for differential diagnosis between dentigerous cyst and unicystic ameloblastoma.