مقايسه درصد حيات و تمايز خود به خودي سلول هاي بنيادي بافت چربي و سلول هاي استرومايي مغز استخوان در پاساژهاي طولاني مدت

Comparison of Adipose-Derived Stem Cells and Bone Marrow Stromal Cells in Prolonged Passages Based on Viability and Auto-Differentiation

سلول هاي بنيادي بافت چربي از نظر ظاهري بسيار شبيه به سلول هاي استرومايي مغز استخوان هستند و ميتوانند جايگزيني مناسب براي اين سلول ها باشند. هدف از اين مطالعه بررسي و مقايسه درصد حيات و تمايز خود به خودي سلول هاي بنيادي بافت چربي و سلول هاي استرومايي مغز استخوان در پاساژهاي طولاني مدت مي باشد. مواد و روش ها در اين مطالعه آزمايشگاهي ابتدا سلول هاي بنيادي بافت چربي و مغز استخوان از موش هاي صحرايي تهيه شد و سپس در پاساژهاي مختلف درصد سلول هاي مرده با روش (live and dead cells assay) و توانايي تمايز خود به خودي سلول ها به سلول هاي چربي ساز و استخوان ساز به ترتيب با استفاده از رنگ آميزي Oil Red O و Alizarin red بررسي شد. اطلاعات به دست آمده توسط Student t-test و روش آماري آناليز واريانس يك طرفه (ANOVA) بررسي گرديد. نتيجه گيري با توجه به درصد سلول هاي مرده و تمايز خودبه خودي در پاساژهاي طولاني مدت، سلول هاي بنيادي بافت چربي مي توانند منبع جايگزين مناسبي براي سلول هاي بنيادي مغز استخوان به منظور كاربرد هاي درماني باشند.

Adipose tissue-derived stem cells (ADSCs) are very similar in their cell properties to bone marrow stromal cells (BMSCs) and can be a suitable alternative for cell therapy trials. The purpose of this study is comparison of adipose-derived stem cells (ADSCs) and bone marrow stromal cells (BMSCs) in prolonged passages based on viability and auto-differentiation toward adipocytes and osteocytes, respectively. Materials an‎d Methods In this experimental study, stem cells were harvested from adipose tissue and bone marrow, then, at different cell passages the percentage of dead cells was assessed by live and dead cells assay and spontaneous differentiation toward adipocytes and osteocytes evaluated using Oil Red O and Alizarin red staining, respectively. T-test and one-way ANOVA statistical analysis was used for assessment of obtained data. Results Attained results of this study showed that the mean percentage of death cells during 5th, 10th and 15th for BMSCs were (2.26±0.36, 3.39±0.45, 6.92±0.95) and for ADSCs were (0.7±0.19, 0.89±0.39, 3.23±0.55), respectively. Also spontaneous differentiation into bone-forming cells of ADSCs and BMSCs in 15th passage shown significant difference for BMSCs (3.25± 0.38) and ADSCs (0.48±0.3) (p< 0.05). Our data show that ADSCs did not spontaneously adipogenic differentiate during 15th passage, but adipogenic differentiation for BMSCs was positive (1.07±0.24). Conclusion Considering of the percentage of dead cells and spontaneous differentiation with long passages revealed that ADSCs can be a suitable alternative source for BMSCs in therapeutic applications.